急性白血病患儿血清铁调素及白细胞介素-6表达的水平分析

Analysis on serum hepcidin and interleukin 6 expression in children with acute leukemia

目的探讨急性白血病患儿血清铁调素及炎性因子白细胞介素-6(IL-6)表达的临床意义。方法选取2012年1月至2017年6月大连医科大学附属大连市儿童医院收治的初诊未治急性白血病患儿73例纳入观察组;另选同期该院健康体检儿童60例纳入对照组。所有研究对象均于清晨空腹采集静脉血,分离血清,采用酶联免疫吸附试验测定铁调素和IL-6水平。结果 38例初诊未治的急性髓性细胞白血病(AML)患儿经首次化疗1个月后,其中32例患儿达到病情缓解,继续化疗6个月后,32例患儿均持续完全缓解。35例初诊未治急性淋巴细胞白血病(ALL)患儿经首次化疗1个月后,其中31例患儿达到病情缓解,继续化疗6个月后,31例患儿均持续完全缓解。AML、ALL组中初诊未治时,首次化疗1个月后缓解和继续化疗6个月缓解的患儿血清铁调素和IL-6水平均高于对照组,差异有统计学意义(P<0.05)。首次化疗1个月后缓解和继续化疗6个月缓解的AML、ALL患儿血清铁调素和IL-6水平均低于初诊未治时,差异有统计学意义(P<0.05)。结论血清铁调素和IL-6水平在一定程度上反映了急性白血病患儿的病情严重程度及治疗效果。

Objective To investigate the clinical significance of serum hepcidin and inflammatory factor interleukin （IL）-6 expression in children with acute leukemia. Methods A total of 73 patients newly diagnosed acute leukemia admitted in Dalian Children′s Hospital of Dalian Medical University from January 2012 to June 2017 were selected as the observation group,meanwhile 60 healthy children undergoing physical examination were selected in the control group.Fasting blood samples were collected in early morning,and serum levels of hepcidin and IL-6 were determined by enzyme linked immunosorbent assay. Results The levels of serum hepcidin and IL-6 in the observation group were higher than those in the control group （ P 〈0.05）.There were 32 cases in 38 untreated acute myeloid leukemia （AML） patients had remission after receiving 1 month chemotherapy,and had completely remission after receiving 6 months chemotherapy.There were 31 cases in 35 untreated cute lymphoblastic leukemia （ALL） patients had remission after receiving 1 month chemotherapy,and had completely remission after receiving 6 months chemotherapy.The levels of serum hepcidin and IL-6 in AML and ALL patients when untreated,remitted after 1 month chemotherapy and completely remitted after 6 months chemotherapy were higher than those in control group （ P 〈0.05）,while serum hepcidin and IL-6 levels at remitted after 1 month chemotherapy and completely remitted after 6 months chemotherapy were significant lower than those when untreated （ P 〈0.05）. Conclusion The expression of serum hepcidin and IL-6 in children with acute leukemia reflects the disease condition and treatment effect to a certain degree.