小檗胺对胃癌细胞增殖、凋亡的影响及其分子机制

Effects of berbamine on cell proliferation and apoptosis in gastric cancer cells and its molecular mechanism

目的:探讨小檗胺对胃癌细胞(AGS和SGC-7901)增殖、凋亡的影响并探讨其分子机制。方法:利用MTT实验检测胃癌细胞的增殖,利用集落形成实验检测胃癌细胞的生长,流式细胞技术检测胃癌细胞的凋亡率以及细胞周期,Western blot检测凋亡相关蛋白的表达水平。结果:小檗胺(0~64μg/ml)能够剂量依赖性以及时间依赖性的抑制AGS和SGC-7901细胞的增殖(P<0.05)。集落形成实验结果显示小檗胺(32μg/ml)能够抑制AGS和SGC-7901细胞的生长(P<0.05)。流式细胞实验显示,小檗胺(32μg/ml)同时能够促进AGS和SGC-7901细胞的凋亡(P<0.05),增殖G_0/G_1期细胞比例,降低S期细胞比例(P<0.05)。Western blot实验结果显示小檗胺(32μg/ml)能够增加AGS和SGC-7901细胞的cleaved caspase-3,cleaved caspase-9以及Bax的蛋白水平,同时降低Bcl-2的蛋白水平(P<0.05)。结论:小檗胺能够抑制胃癌细胞的增殖,其机制可能与改变细胞周期以及促进细胞凋亡有关。

Objective：To investigate the effects of berbamine on the cell proliferation in gastric caner cell lines （AGS and SGC-7901 cells）,to explore its underlying mechanisms.Methods：Cell proliferation was determined by MTT assay.Cell growth was determined by colony formation assay.Flow cytometry was used to determine cell apoptosis and cell cycle,Western blot was used to determine apoptosis-related protein levels.Results：Berbamine （0~64 μg/ml） suppressed AGS and SGC-7901 cell proliferation in a concentration- and time-dependent manner （P＜0.05）.Colony formation assay showed that berbamine （32 μg/ml） suppressed AGS and SGC-7901 cell growth （P＜0.05）.Flow cytometry analysis showed that berbamine （32 μg/ml） induced apoptosis,and increased cell population at G0/G1 phase and decreased the cell population at S phase in AGS and SGC-7901 cells （P＜0.05）.Western blot results showed that berbamine （32 μg/ml） increased the protein levels of cleaved caspase-3,cleaved caspase-9 and Bax,and decreased the protein level of Bcl-2 in AGS and SGC-7901 cells （P＜0.05）.Conclusion：Berbamine suppressed cell proliferation in gastric cancer cells,possibly via inducing apoptosis and modulating cell cycle.