氯化十六烷基吡啶-肝素体系的共振瑞利散射光谱及其应用

Resonance rayleigh scattering of cetylpyridinium chloride-heparin system and its application

目的基于肝素（Hep）对氯代十六烷基吡啶（CPCM）共振瑞利散射的增强作用，建立新的测定肝素的方法。方法在pH4．5～6．5缓冲溶液中，Hep—CPCM体系有一灵敏的散射光谱，最大散射峰在293nm处，在542nm处有一个较弱的吸收峰。结果0．04～1．2mg·L^-1Hep与散射强度符合线性关系，方法的检出限为0．011mg·L^-1。结论所用方法简便、检出限低，可用于注射液中Hep的测定。

OBJECTIVE To develop a novel method for the determination of heparin based on the enhancement of Resonance rayleigh scattering （RRS） of cetylpyridinium chloride in the presence of heparin. METHODS In pH4.5 - 6.5 britton - robinson buffer solution ,the RRS signal of heparin and cetylpyridinium chloride alone was very weak,but it was etahaneed greatly and a new RRS spectra appeared when they interacted with each other to form the aion - association complex. The strong peak was at 293 nm and the weak peak was at 542 nm. RESULTS The mass concentration of heparin in the range of 0.04 - 1.2 mg· L^- 1 was linear to the intensity of RRS. The detection limit was 0.011mg· L^- 1 CONCLUSION The method is simple, it has low detection limit and can determine the heparin sodium in injections with satisfactory results.