HPLC同时测定中药材中的5种脂溶性红色素

Simultaneous determination of five fat-soluble red pigments in Chinese medicinal materials by HPLC

目的采用HPLC法同时检测脂溶性红色素（苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ、颜料橙5）的含量，并用于检测中药及饮片非法添加的色素。方法选用资生堂CapcellPAKC18 ACR色谱柱（250mm×4．6mm，5μm），以乙腈-水为流动相进行梯度洗脱，流速1．0mL·min^-1，柱温为30℃，在不同波长下（480、508nm）进行检测。结果1～50μg·mL^-15种脂溶性红色素的线性关系良好，精密度、稳定性的RSD均小于5％，加样回收率为80％～120％。结论所用方法操作简单、快速、准确、重复性好、灵敏度高，可用于筛查中药材及饮片中脂溶性红色素的非法染色。

OBJECTIVE To establish a method of HPLC for simultaneously detecting fat - soluble red pigments （ sudan Ⅰ , sudan Ⅱ , sudan Ⅲ, sudan Ⅳ, pigment orange 5 ） , and to detect illegally added pigments in Chinese medicinal materials and pieces. METH- ODS The Shiseido CAPCELL PAK C18 column （250 mm × 4.6 ram,5 μm）was used to separate the samples at 30 ℃ with a step linear gradient using acetonitrile -water at a flow rate of 1 mL·min^-1 ,and detection was performed at different wavelengths （480,508 nm）. RESULTS Five kinds of fat - soluble red pigments had good linear relationship in the range of 1 - 50 μg· mL^- 1, the RSD of precision and stability were less than 5% ,and the recovery was between 80% and 120%. CONCLUSION The method is simple, rapid and accurate with good reproducibility and high sensitivity, and it is suitable for the screening of the illegal dyeing of fat - soluble red pigments in traditional Chinese medicine and herbs.