砷冶炼厂工人肿瘤相关基因mRNA表达变化

Expression changes of tumor-related gene mRNA in workers who smelt arsenic

目的探讨砷冶炼厂工人肿瘤相关基因mRNA表达变化规律及影响因素。方法选择在岗砷冶炼工人37名、停止砷冶炼85天的工人43名和对照组51名为研究对象,使用带砷预处理系统的原子吸收分光光度法检测研究对象尿中无机砷、甲基胂酸和二甲基胂酸含量;实时荧光定量聚合酶链式反应(PCR)检测研究对象外周血Lin28、Bax、Bcl-2、Fas基因的mRNA相对表达量。结果尿中无机砷、甲基胂酸和二甲基胂酸浓度分别为:在岗砷冶炼工人(133.97±109.53)、(208.93±171.43)和(820.35±487.39)μg/g肌酐;停止砷冶炼工人(123.31±112.72)、(176.21±157.19)和(467.73±392.17)μg/g肌酐;对照组(1.55±1.49)、(0.10±0.09)和(10.47±7.85)μg/g肌酐。砷接触组工人尿中3种砷化合物浓度均显著高于对照组,正在进行砷冶炼的工人二甲基胂酸浓度显著高于停止砷冶炼工人(P<0.05)。研究对象相应外周血Lin28、Bax、Bcl-2、Fas基因的mRNA相对表达量分别为:在岗砷冶炼工人(8.88±2.42)、(6.87±1.10)、(7.24±2.31)和(8.23±2.90);停止砷冶炼工人(6.21±2.94)、(5.81±1.72)、(4.50±1.59)和(6.89±2.35);对照组(5.60±1.43)、(5.56±0.98)、(4.88±1.39)和(6.92±1.87)。在岗砷冶炼工人Lin28、Bax、Bcl-2、Fas基因的mRNA相对表达量均显著高于对照组(P<0.05)。结论砷冶炼工人多种肿瘤相关基因mRNA高表达,无机砷甲基化代谢转化在相关mRNA高表达中具有重要作用。

Objective To explore the changes and influencing factors about expressions of tumor-related gene mRNA in workers who smelt arsenic.Methods There were 37 workers who smelt arsenic,43 workers who stopped exposure to arsenic for 85 days,and 51 individuals as control group which selected by random cluster sampling.Arsenic species（ iAs,MMA,and DMA） in urine were determined by atomic absorption spectrophotometer with an As speciation pretreatment system.Real time PCR（ RT-PCR）was performed to detect the expressions of 4 tumor-related gene mRNAs.Results The concentrations of iAs,MMA and DMA in urine of workers who smelt arsenic,stopped exposure to arsenic for 85 days,and control group were（ 133.97 ± 109.53）,（ 208.93 ±171.43） and（ 820.35 ± 487.39） μg/（ g·creatinine）（ workers who smelt arsenic）,（ 123.31 ± 112.72）,（ 176.21 ± 157.19） and（ 467.73 ± 392.17） μg/（ g·creatinine）（ workers who stopped exposure to arsenic）,（ 1.55 ±1.49）,（ 0.10 ±0.09） and（ 10.47 ±7.85） μg/（ g·creatinine）（ control group）.Compared to control group,the concentrations of 3 arsenic species were all higher in worker came from arsenic smelting.Compared to workers who were smelting arsenic,DMA are lower in workers who stopped exposure to arsenic for 85 days（ P 〈 0.05）.The relative mRNA expressions of Lin28,Bax,Bcl-2 and Fas in 3 groups were（ 8.88 ± 2.42）,（ 6.87 ± 1.10）,（ 7.24 ± 2.31） and（ 8.23 ±2.90）（ workers who smelt arsenic）,（ 6.21 ± 2.94）,（ 5.81 ± 1.72）,（ 4.50 ± 1.59）and（ 6.89 ± 2.35）（ workers who stopped exposure to arsenic）,（ 5.60 ± 1.43）,（ 5.56 ±0.98）,（ 4.88 ± 1.39） and（ 6.92 ± 1.87）（ control group）.Compared to control group,relative mRNA expressions of Lin28,Bax,Bcl-2 and Fas were all higher in worker who were smelting arsenic（ P 〈 0.05）.Conclusion The expressions of tumor-related gene mRNAs are high in workers who smelt arsenic,and the methylation metabolism of arsenic play great role in the process of relative mRNAs expresses.