重复经颅磁电刺激对海人酸致痫鼠的疗效研究

Therapeutic effect of repetitive transcranial magnetic therapy on kainic acid induced epileptic rats

目的探讨经颅磁电刺激（TMES）疗法对海仁酸（KA）致颞叶癫痫鼠的疗效。方法62只大鼠按照随机数字表法分为预处理组（32只）和治疗组（30只），预处理组再分为4个小组，分别给予治疗仪最大电流强度（MCI）0％，25％，50％，75％的电流刺激，确定本实验条件下的最佳刺激参数。治疗组再分为3个小组，每组10只。2组（癫痫刺激组、癫痫非刺激组）为符合纳入标准的癫痫模型大鼠，刺激组刺激参数为预处理组确定出的最佳刺激参数；非刺激组大鼠刺激前后处理与刺激组相同，但治疗仪无有效的能量输出。第3组对照组为未造模对照大鼠。3组大鼠均给予每天1次，40min／次，共14d的刺激。记录并比较3组大鼠行为学、电生理学及组织学变化，以评估TMES疗法对癫痫大鼠的疗效。结果50％MCI为最佳刺激强度。癫痫刺激组大鼠癫痫波出现的频率明显低于癫痫非刺激组大鼠[（30．210±4．580）次／min vs．（31．380±4．247）次／min]，差异有统计学意义（t=3．325，P=0．001）。Timm染色结果提示3组大鼠染色评分差异有统计学意义（F=17．429，P=0．000），癫痫刺激组大鼠海马齿状回的内分子层Timm染色评分较癫痫非刺激组明显减轻。差异有统计学意义（P〈O．05）。结论TMES疗法可通过改善癫痫大鼠海马齿状回内分子层组织学变化程度来影响齿状回神经元环路的形成，进而降低癫痫脑电波的发作频率。

Objective To investigate the effect of transcranial magnetoelectric stimulation （TMES） on temporal lobe epilepsy induced by kainic acid （KA） in rats. Methods Sixty-two rats were randomly divided into pretreatment group （n=32） and treatment group （n=30）; rats in the pretreatment group were divided into 4 subgroups, giving TMES at 0%, 25%, 50%, and 75% maximum current intensity （MCI）, respectively, to conform the optimal TMES current. Rats in the treatment group were divided 3 subgroups （n=10）： TMES epilepsy group （giving TMES at optimal current）, non-TMES epilepsy group, and control group; TMES was given 40 min/once daily for 14 d; the behavior, histological and electrophysiological changes of rats in the 3 groups were recorded, and the treatment efficacy of TMES in epileptic rats were evaluated. Results The optimal current was 50% MCI. The frequency of epileptic waves in TMES epilepsy group was significantly lower than that in non-TMES epilepsy group （[30.210± 4.580] times/min vs. [31.380±4.247] times/min, t=3.325, P=0.001）. Timm staining results indicated significant differences among the three groups （F=17.429, P=0.000）, and the degree of Timm staining in the inner molecular layer of hippocampus dentate gyrus in the TMES epilepsy group was significantly slighter than that in the non-TMES epilepsy group （P〈0.05）. Conclusion TMES can influence the formation of dentate gyrus neurons loop by improving molecular layer histological changes in epileptic rats, thereby reducing frequency of epileptic EEG seizures.