B细胞分离方法的研究进展

A review on B cell isolation methods

B细胞是重要的免疫细胞,如何筛选和分离分泌抗体的抗原特异性B细胞,对于研制一些特殊功能的抗体具有重要的方法学意义。本文阐述了不同发育阶段B细胞的表面分子特征与种属差异,以及基于这些表面分子的不同B细胞分离方法的原理与优缺点,旨在为抗原特异性B细胞的分离提供参考。

B cell is an important immune cell.How to screen and isolate antigen-specific B cells that secrete antibodies has important methodological significance for the development of antibodies with special functions.In this review,B cell surface molecule characteristics and species differences at different developmental stages were reviewed,the principles,advantages and disadvantages of different B cell separation methods based on these surface molecules were also described.This paper may provide a useful reference for the separation of antigen-specific B cells.