摘要
B细胞是重要的免疫细胞,如何筛选和分离分泌抗体的抗原特异性B细胞,对于研制一些特殊功能的抗体具有重要的方法学意义。本文阐述了不同发育阶段B细胞的表面分子特征与种属差异,以及基于这些表面分子的不同B细胞分离方法的原理与优缺点,旨在为抗原特异性B细胞的分离提供参考。
B cell is an important immune cell.How to screen and isolate antigen-specific B cells that secrete antibodies has important methodological significance for the development of antibodies with special functions.In this review,B cell surface molecule characteristics and species differences at different developmental stages were reviewed,the principles,advantages and disadvantages of different B cell separation methods based on these surface molecules were also described.This paper may provide a useful reference for the separation of antigen-specific B cells.
作者
王省
李坤
卢曾军
刘在新
曹轶梅
WANG Sheng;LI Kun;LU Zeng-jun;LIU Zai-xin;CAO Yi-mei(Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Etiological Biology/National Foot-and-Mouth Disease Reference Laboratory,Lanzhou 730046,Chin)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2018年第8期1031-1037,共7页
Chinese Veterinary Science
基金
国家自然科学基金面上项目(31372422)
关键词
B细胞
分离方法
抗体
B cell
isolation methods
antibody