血清黄体生成素与卵泡刺激素比值及雄激素受体基因多态性与多囊卵巢关系的探讨

Correlation between ratio of LH and FSH,the androgen receptor gene polymorphism and PCOM

目的探讨血清黄体生成素(LH)与卵泡刺激素(FSH)比值在多囊卵巢(PCOM)患者中的参考价值;探讨雄激素受体(AR)基因rs6152G/A多态性与PCOM的关系。方法收集2016年6—12月在贵州医科大学附属医院生殖中心就诊的PCOM患者共57例,无PCOM的不孕患者34例,记录患者B超下双侧卵泡数目及基础性激素值,并提取全血DNA,用聚合酶链反应法(PCR)扩增AR基因含rs6152基因位点的片段并测序。结果(1)PCOM组较对照组患者年龄偏小、窦卵泡数(AFC)明显增多,睾酮值增加,差异有统计学意义(P<0.01);将LH/FSH比值进行分组比较,当LH/FSH≥1时,睾酮值和AFC数较LH/FSH<1组明显增加,差异有统计学意义(P<0.01)。(2)随着LH/FSH比值的增高,携带易患高雄激素血症(HA)AR基因rs6152A基因型的患者人数增加,但相关趋势无统计学意义。结论 (1)LH/FSH≥1是PCOM患者提前干预的重要指标,可能会减少多囊卵巢综合征(PCOS)的发生。(2)携带AR基因rs6152A基因型的患者易出现PCOM。

Objective To estimate the value of LH/FSH in patients with PCOM and find out the correlation between polymorphism of AR gene rs6152 G/A and PCOM.Methods Data was collected from 57 PCOM patients and34 infertility women without PCOM.It was recorded the number of bilateral follicular and the base sex hormone values under the ultrasound and collected the whole blood DNA.Run PCR on the AR gene rs6152 G/A.Results PCOM group was younger than the control T group but showed a significant increase（P〈0.01）in Antral follicle（AFC）and Testosterone.When LH/FSH≥1,values of Antral follicle（AFC）and Testosterone were significantly higher（P〈0.01）than when LH/FSH1.With the increasing value of LH/FSH,there were more patients positive with AR gene rs6152 G/A gene,but the deference was not significant.Conclusion LH/FSH≥1 is an important index for patients with PCOM to prevent from PCOS.Patients are positive with AR gene rs6152 G/A gene have a higher tendency to develop and PCOM,and thus develop PCOM.