天青A-BSA共振散射光谱研究及分析应用

Study of resonance light scattering of Azure A-BSA and its analytical application

考察了天青A与牛血清白蛋白(BSA)混合体系的共振散射光谱,发现天青A的加入能引起BSA的共振散射光谱强度明显增强,通过优化实验条件建立了测定蛋白的新方法.在p H=3.78的磷酸氢二钠-柠檬酸缓冲溶液中,固定天青A浓度为1.00×10-5mol/L,当牛血清白蛋白浓度在8.80×10-8~4.40×10-6mol/L范围内与体系的共振光散射强度值呈良好的线性关系,线性方程为IRLS=69.744c(mol/L)+59.359,相关系数r=0.992 8,检出限为3.28×10-8mol/L,方法应用于牛尿样品中白蛋白含量的测定,回收率在97%~106%之间,表明方法具有较高的准确度,结果令人满意.

The resonance light scattering of azure A-BSA was investigated. The intensity of resonance scattering spectrum was obviously enhanced when 1.00×10-5 mol/L Azure A was added. A new method of determination of protein was established by optimization of the experimental conditions of resonance scattering spectroscopy of azure A and BSA. In disodium hydrogen phosphate of p H = 3. 78,citrate buffer solution resonance light scattering（ RLS） intensity showed a good linear relationship in the concentration range of BSA with 8. 80 × 10-8-4.40 × 10-6 mol/L. The linear equation was IRLS= 69. 744 c（mol/L）＋59.359,and the detection limit was 3.28×10-8 mol/L. Applied to the determination of BSA in bovine urine samples,the recovery was at the range of 97% to 106%,and satisfactory results was obtained.