索拉非尼联合盐霉素对肾癌细胞KCC853体外增殖的影响

Effects of sorafenib and salinomycin on the proliferation of renal cancer cell KCC853

目的探讨索拉非尼与盐霉素联合应用对肾癌细胞KCC-853体外增殖的影响。方法 MTT法检测药物单用及不同比例合用对肿瘤细胞生长的抑制,应用Excel 2016和ORIGIN 8.0处理数据,拟合得出各用药组量效曲线及函数方程,loewe等效线模型法计算合用组抑制率为ED30、ED50、ED70、ED90时的合用指数(CI),评价联合用药效应。流式细胞检测受试药物对KCC853细胞凋亡的影响。结果盐霉素∶索拉非尼(盐∶索)=2∶1合用,抑制率为ED30、ED50、ED70和ED90时的CI分别为1.195 4、1.096 2、1.054 0、0.957 3;盐∶索=1∶1合用,抑制率为ED30、ED50、ED70和ED90时的CI分别为0.797 2、0.854 8、0.944 3、1.037 2;盐∶索=1∶2合用,抑制率为ED30、ED50、ED70和ED90时的CI分别为0.844 0、0.821 2、0.849 2、0.932 8。盐霉素与索拉非尼能明显促进KCC853细胞凋亡,凋亡细胞比例分别为49.03%、48.60%;联合用药组凋亡细胞比例达60.57%(P<0.05)。结论索拉非尼与盐霉素按上述比例联合使用表现出良好的相加和协同效应,盐霉素可以增强索拉非尼对KCC853细胞增殖的抑制作用及其诱导KCC853细胞凋亡的作用。

Objective To investigate the effect of salinomycin（sal）combined with sorafenib（sor）on proliferation of human renal cancer cell line KCC853. Methods MTT assay was applied to assess the anti-proliferative effect.Excel 2016 andORIGIN 8.0 software were used to analyze the data.The drug concentration of the single drug group and combined drug groupwere measured when the inhibitory effect reached ED30,ED50,ED70,ED90,the combination index（CI）value was calculated withloewe isobologram method for each dose level and evaluted the combination effect.Flow cytometry was used to assess the extentof cell apoptosis. Results The CIs of ED30,ED50,ED70,ED90 in the sal∶sor=2∶1 were 1.195 4,1.096 2,1.054 0,0.957 3;theCIs of ED30,ED50,ED70,ED90 in the sal∶sor=1∶1 were 0.797 2,0.854 8,0.944 3,1.037 2;the CIs of ED30,ED50,ED70,ED90 inthe sal∶sor=1∶2 were 0.844 0,0.821 2,0.849 2,0.932 8.sal and sor could promote apoptosis of KCC853 cells,the proportion ofapoptotic cells were 49.03% and 48.60%;combined drug group reached 60.57%（P0.05）. Conclusion Combined sal and sorat the above rates shows good additive and synergistic effects,sal can enhance the inhibitory effect and induce apoptosis of sor on KCC853 cells.