改进Dische比色法测定海带中的岩藻聚糖含量

The improvement of Dische colorimetric method in the determination of fucoidan in seaweed

采用Dische比色法测定海带中的岩藻聚糖含量,具有操作简便,不需要昂贵仪器,安全可靠等特点,但分析速度慢。为了提高测定方法的效率,通过考察振荡条件、温度、L-半胱氨酸盐添加量对显色稳定性的影响,对Dische比色法进行改进。并验证方法学,考察了精密度、显色稳定性、加标回收率。实验表明,改进Dische比色法大大缩短显色时间,精密度、稳定性、加标回收率符合相关规定,并采用改进的Dische法测定不同地区12种海带中岩藻聚糖含量。

The content of fucoidan in seaweed can be determined by Dische colorimetry. It is a simple methods,easily operating without expensive equipment,and is safe and reliable. But the speed of the analysis is too slow. The effects of the shaking conditions,temperature and L-cysteine hydrochloride on the color stability were investigated in this study to improve the efficiency of the method. Verification methodology was applied by investigating the precision,color stability and spike and recovery. The experimental results show that the modified Dische colorimetry can greatly shorten color reaction time,and improve the precision,color stability,and spike and recovery are in accordance with the relevant regulations. The contents of fucoidan in different kind of seaweed were determined by the modified Dische colorimetry.