桑葚中烯酰吗啉残留量测定

Determination of Dimethomorph Residues in Mulberry

建立了检测桑葚中烯酰吗啉残留量的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。桑葚样品经乙腈提取,石墨化炭黑/氨基复合柱(Carbon/NH_2)净化,以0.1%甲酸甲醇溶液+0.1%甲酸水溶液+0.1%甲酸乙腈溶液为流动相进行梯度洗脱,采用电喷雾正离子模式,多反应监测方式进行采集,外标法定量。烯酰吗啉在桑葚中的检测限为0.25μg/kg,定量限为1.00μg/kg。在1.00~50.00μg/L质量浓度范围内,方法线性关系良好,相关系数(R^2)为0.999;方法的回收率(92.18%~104.39%)和精密度(3.48%~5.13%)均满足定量分析要求。该方法适用于桑葚中烯酰吗啉残留量的测定。

An ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） method for quantification of dimethomorph in mulberry was developed. The mulberry samples were extracted by acetonitrile, purified by Carbon/NH2 column. The residues of dimethomorph were determined by multiple reaction monitoring mode with positive electrospray ionization,and quantified by the method of external standard. The results showed that at the concentration of 1.00-50.00 μg/L, the method had good linear relationship, with the correlation coefficient （R2） of 0.999. The limit of detection was 0.25 μg/kg, and the limit of quantification was 1.00 μg/kg. The average recoveries （92.18%- 104.39%） and precision （3.48%-5.13%） of this method could meet the requirements of analytical method. This method was suitable for the determination of dimethomorph residues in mulberry.