凝胶样支架材料对三维培养的人牙髓干细胞增殖的影响

Effects of gel-like scaffold on proliferation of human dental pulp stem cells

目的目的评价牙髓组织工程中不同浓度的两种可注射性凝胶样支架材料对牙髓干细胞增殖的影响。方法配制不同浓度的Ⅰ型胶原、肽段水凝胶(Puramatrix)支架材料,将牙髓干细胞分别接种于各组支架材料上;CCK8方法检测细胞增殖情况;活死细胞试剂盒染色观察细胞数量及形态。结果接种在各浓度支架材料上的牙髓干细胞均生长良好,其中1%的Ⅰ型胶原支架和0.25%的肽段水凝胶支架对牙髓干细胞的增殖有明显的促进作用;活死细胞染色观察牙髓干细胞在各浓度的两种支架材料中均生长良好,活细胞数量随培养时间的延长呈递增趋势;细胞充分伸展,呈纺锤型。结论适于牙髓干细增殖的Ⅰ型胶原及肽段水凝胶(Puramatrix)支架浓度分别为1%及0.25%。

Objective To evaluate the effects of two kinds of injectable gel-like scaffolds with different concentrations on the proliferation of dental pulp stem cells in dental pulp tissue engineering. Methods Different concentrations of type Ⅰ collagen and peptide hydrogel scaffolds were prepared,and dental pulp stem cells were inoculated on each group of scaffolds respectively. Cell proliferation was detected by CCK8 assay; live/dead cell staining was used to observe the number and morphology of cells. Results The dental pulp stem cells inoculated with different concentrations of scaffolds all grew well. 1% collagen scaffolds and 0.25% hydrogel scaffolds could promote the proliferation of dental pulp stem cells. Live/desd cell staining showed that dental pulp stem cells on both scaffold materials with various concentrations grew well,and the live number of cells showed an increasing trend with the prolongation of culture time. The cells were fully extended and showed a spindle type. Conclusion The concentrations of type Ⅰ collagen and Puramatrix hydrogel scaffolds suitable for dental pulp stem cell proliferation are respectively 1% and 0.25%.