Foxp3^+Treg及免疫抑制因子PD-L1在宫颈病变微环境中的表达

Expressions of negative regulator Foxp3^+Treg and PD-L1 in the immune microenvironment of cervical cancer

对比Foxp3^+Treg与免疫抑制因子PD-L1在正常和不同病变状态下的宫颈微环境中的表达情况。采用亲和免疫组织化学技术SP法分别检测子宫颈癌(cervical cancer,CC)组织64例,宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)组织50例和正常宫颈组织50例,对比宫颈组织中Foxp3^+Treg和PD-L1的表达结果。宫颈病变组织中的Foxp3^+Treg和PD-L1蛋白表达均明显高于正常组织(P<0.05);随着病理级别升高,Foxp3^+Treg的侵袭性和PD-L1蛋白在异常分化细胞中的表达增强(P<0.05);Foxp3^+Treg与异常分化细胞中PD-L1的表达呈显著正相关(r=0.359,P=0.002)。在CC不断进展后,从不同组织病理阶段上看,宫颈细胞发生持续恶性转化的同时宫颈局部微环境也发生改变,即其中的Foxp3^+Treg数量在不断增多,且异常分化细胞中PD-L1的表达也逐渐增强。

The expressions of Foxp3^＋Treg and PD-L1 in the cervical microenvironment under normal and different pathological conditions were compared.Affinity immunohistochemistry SP method was used to detect and compare the expression of Foxp3^＋Treg and PD-L1 in 50 cases of cervical intraepithelial neoplasia（CIN）,64 cases of cervical cancer（CC）and 50 normal cervical tissues.The expressions of Foxp3^＋Treg and PD-L1 in cervical lesions were significantly higher than those in the normal tissues（P〈0.05）.With the progress of pathological grade,the invasiveness of Foxp3^＋Treg and the expression of PDL1 in abnormally differentiated cells elevated（P〈0.05）;Foxp3^＋Treg was positively correlated with PD-L1 expression significantly in abnormally differentiated cells（r=0.359,P =0.002）.Cervical microenvironment changed with progressioon of CC,as judged from the pathological staging,the number of Foxp3^＋Treg cells was increased and PD-L1 expression in abnormally differentiated cells was increased.With continuous progression of malignant transformation of cervical cells,the cervical microenvironment changed,as evidenced by increased number of Foxp3^＋Treg cells and gradually enhanced expression of PD-L1 in abnormally differentiated cells.