金属硫蛋白1X在非小细胞肺癌中的表达及临床意义

Expression of metallothionein 1X in non-small cell lung cancer and its significances

目的:探讨金属硫蛋白1X(metallothionein 1X,MT1X)在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者的癌组织与癌旁组织中表达及临床意义,分析MT1X与NSCLC预后的相关性。方法:纳入68例肺癌患者,采用实时定量PCR(RT-PCR)法及免疫组织化学(IHC)方法检测NSCLC患者癌与癌旁组织中MT1X m RNA及蛋白的表达情况。利用网上数据库(http://kmplot.com/analysis/)分析MT1X与NSCLC患者生存的相关性。结果:RT-PCR结果显示MT1X m RNA在癌组织中的相对表达量明显高于癌旁组织,差异有统计学意义(0.057±0.013 vs 0.011±0.008,P<0.01);免疫组织化学结果显示MT1X在NSCLC中的阳性表达率为67.6%(46/68),明显高于癌旁组织的39.7%(27/68)。MT1X表达水平在NSCLC患者的不同p TNM分期、分化、有无脉管侵袭、有无淋巴结转移间存在显著差异,而与患者的性别、年龄、肿瘤大小、组织学类型、肿瘤位置无关。Kaplan-Meier生存分析结果显示MT1X高表达与NSCLC患者总生存期短显著相关(P<0.001)。结论:MT1X在NSCLC患者中阳性表达与患者术后生存期短显著相关,可作为评估NSCLC患者预后的重要标志分子。

Objective： To investigate the expression of metallothionein 1 X（MT1 X） in human non-small cell lung cancer（NSCLC）, and its correlation with clinical significance as well as prognosis. Methods： A total of 68 patients with NSCLC were enrolled, the expression of MT1 X m RNA and protein in tumor tissue and adjacent normal tissue were detected by real-time PCR and immunohistochemistry（IHC）. The relationship between MT1 X expression and clinicopathological features were statistically analyzed. Kaplan-Meier survival analysis for the relationship between survival time and MT1 X signature in NSCLC was done with an online tool（http：//kmplot.com/analysis/）. Results： The m RNA expression of MT1 X in NSCLC was 0.057±0.013, which was higher than that in adjacent normal tissues（0.011±0.008, P〈0.01）. The expression of MT1 X protein in NSCLC was also higher than that in adjacent tissues（67.6% vs 39.7%, P〈0.01）. In addition, MT1 X expression had significant difference in p TNM, lymph node metastasis and poor survival time, but it did not show related with age, gender, histology, tumor size and the location of tumors. Conclusion： MT1 X is frequently upregulation in NSCLC, and its overexpression is significantly correlated with poor prognostic, suggesting that MT1 X could be used as a potential therapeutic target for the prevention and treatment of NSCLC.