电针对炎性痛大鼠镇痛作用的观察

Electrical Acupuncture Efficacy on Inflammatory Pain

目的:观察电针对完全弗氏佐剂诱导的炎性痛大鼠的镇痛作用,并探讨其相关机制。方法:将30只SD雄性大鼠按随机抽样法分为3组,生理盐水+假电针组(NS+SE)、完全弗氏佐剂+假电针组(CFA+SE)、完全弗氏佐剂+电针组(CFA+EA),每组10只。于造模第4天和治疗前,分别用Von Frey法和PWL法测定大鼠机械痛和热敏痛,测痛后治疗30 min,治疗后再次测痛;造模第5天和治疗前1.5 h分别在大鼠右侧足三里穴位附近胫骨前肌处用微透析开始收集透析液直至治疗后1.5 h,收集治疗前30 min、治疗中30 min、治疗后30、60、90 min的组织液,并用高效液相仪分析其腺苷的水平。结果:造模前3组大鼠痛阈差异无统计学意义(P>0.05),造模后CFA+SE组、CFA+EA组的痛阈明显低于NS+SE组(P<0.05)。电针治疗后,与CFA+SE组相比,CFA+EA组痛阈显著增高(P<0.05),Von Frey值与PWL值均明显延长(P<0.05)。与NS+SE组、CFA+SE组相比,CFA+EA组在治疗后足三里穴位局部的腺苷水平明显提高(P<0.05),但是NS+SE组与CFA+SE组治疗前后比较,足三里穴位局部的腺苷水平差异无统计学意义(P>0.05)。结论:电针能有效降低完全弗氏佐剂所致炎性痛大鼠对机械痛和热敏痛的反应,缓解炎性痛,其镇痛机制可能通过增高治疗局部周围的组织液中的腺苷而抑制炎性痛。

Objective：To explore electrical acupuncture（EA） therapy effects on complete freund＇s adjuvant（CFA） induced inflammatory pain model rats. Methods：A total of 30 SD male rats were randomly divided into normal saline ＋ Sham Electroacupuncture（NS＋SE） group, CFA＋Sham EA（CFA＋SE） group and CFA＋ Electroacupuncture（CFA＋EA） group, with 10 rats in each group.Mechanical and thermal allodynia were assessed before and after EA treatment at Day 4 after CFA injection. Dialysate of the rats was collected from the right tibialis anterior muscle by microdialysis at Day 5 from 1.5 hours before the treatment until 1.5 hours after treatment. Adenosine concentration in the dialysate was analyzed by HPLC. Results：At Day 4 after injection of paclitaxel, EA treatment can obviously increase the pain threshold of CFA-induced inflammatory pain rat model. The mechanical allodynia showed that the Von Frey result of the CFA＋EA group was significantly decreased（P〈0.05） after treatment, and the PWL results also significantly prolonged（P〈0.05） compared with the NS＋SE group and the CFA＋SE group. Conclusion ：EA can increase rats pain threshold of CFA-induced inflammatory pain, and alleviate inflammatory pain. The alleviate of inflammatory pain might through adenosine increasment in the local tissue fluid around the acupoints.