Mcl-1 siRNA转染对EC9706细胞紫杉醇化疗敏感性的影响

Effect of Mcl-1 siRNA transfection on sensitivity of EC9706 cells to paclitaxel

目的:观察Mcl-1 siRNA转染后食管鳞癌EC9706细胞对紫杉醇敏感性的变化。方法:应用Western blot方法检测正常食管上皮Het-1细胞及EC9706细胞中Mcl-1蛋白的表达。采用不同浓度的紫杉醇分别处理转染和未转染Mcl-1 siRNA的EC9706细胞48 h,应用MTT法观察细胞增殖能力的变化;采用0.4μmol/L紫杉醇处理转染和未转染Mcl-1 siRNA的EC9706细胞24 h,用流式细胞术检测细胞凋亡。结果:与Het-1细胞相比,EC9706细胞中Mcl-1蛋白高表达。与单用紫杉醇处理的EC9706细胞相比,Mcl-1 siRNA转染联合紫杉醇处理的EC9706细胞增殖抑制率升高,凋亡率也升高(P<0.05)。结论:干扰抗凋亡基因Mcl-1可以增强EC9706细胞对紫杉醇的敏感性。

Aim： To observe the sensitivity of esophageal cancer EC9706 cells to paclitaxel（ PTX） after Mcl-1 RNA interference. Methods： Western blot was used to detect the expression of Mcl-1 protein in normal esophageal epithelial Het-1 cells and esophageal carcinoma EC9706 cells. EC9706 cells transfected with or without Mcl-1 siRNA were treated with different concentrations of PTX for 48 h,and the changes in cell proliferation were observed by MTT assay. EC9706 cells transfected with or without Mcl-1 siRNA were treated with 0. 4 μmol/L PTX for 24 h,and the apoptosis was examined by flow cytometry. Results： Compared with Het-1 cells,Mcl-1 protein was highly expressed in EC9706 cells. Compared with the EC9706 cells treated by PTX alone,those treated by PTX combined with Mcl-1 siRNA transfection were more sensitive to PTX with higher proliferation inhibition rate and apoptosis rate（ P〈0. 05）. Conclusion： The interference of antiapoptosis gene Mcl-1 can increase the sensitivity of EC9706 cells to PTX.