细胞黏附分子1在子宫内膜异位症患者中的表达差异

Different expression level of cell adhesion molecule 1 in cases of endometriosis

目的探讨细胞黏附分子1(CADM1)在子宫内膜异位症患者异位内膜、在位内膜及正常子宫内膜组织中的表达差异。方法 2016年9月至2017年6月在广东省佛山市第五人民医院获取子宫内膜异位症患者异位内膜、在位内膜各20例及正常子宫内膜20例,采用免疫组化染色、Western Blotting分别检测3组内膜组织中CADM1表达水平。结果异位内膜细胞CADM1染色阳性表达最低[(37.80±6.21)%],在位内膜细胞次之[(58.40±8.32)%],正常内膜细胞中最高[(95.20±13.20)%],差异有统计学意义(F=4.97,P=0.028);CADM1蛋白相对表达量在异位内膜中最低(2.10±0.10),在位内膜中次之(2.80±0.20),正常内膜中最高(3.70±0.20),差异有统计学意义(F=10.12,P=0.017)。结论 CADM1基因在子宫内膜异位症患者异位内膜及在位内膜中低表达,其表达缺失可能与病变内膜的转移性生长具有相关性。

Objective To investigate the different expression level of cell adhesion molecule 1（CADM1）in ectopic endometrium and eutopic endometrium of patients with endometriosis（Ems）and normal endometrium.Methods Totally 20 cases of ectopic endometrium and eutopic endometrium from Ems patients as well as 20 cases of normal endometrium were collected.Immunohistochemical staining and Western Blotting were used to detect the expressions of CADM1 in endometrial tissues of three groups at cell level and protein level.Results Positive expression of CADM1 staining was lowest in ectopic endometrial（37.80±6.21%）,followed by that in eutopic endometrium（58.40±8.32%）,and it was highest in normal endometrial cells（95.20±13.20%）.The difference was statistically significant（F=4.97,P=0.028）.The relative expression of CADM1 protein was lowest in ectopic endometrial（2.10±0.10）,followed by that in eutopic endometrium（2.80±0.20）,and it was highest in normal endometrial cells（3.70±0.20）.The difference was statistically significant（F=10.12,P=0.017）.Conclusion CADM1 gene is low expressed in ectopic endometrium and eutopic endometrium of endometriosis patients,and its expression deletion might be related to the metastatic growth of endometrial lesions.