某品牌保肝胶囊对酒精性肝损伤保护作用的研究

Study on the Protective Effect of a Hepatoprotective Capsule Against Alcoholic Liver Injury

目的深入研讨某品牌保肝胶囊在保护乙醇致酒精性肝损伤大鼠中的作用。方法运用酒精肝损伤模型法,每日向受试物3个剂量组灌胃不同剂量受试物,阴性对照组和模型对照组每日灌胃蒸馏水。除阴性对照组外,受试物3个剂量组和模型对照组在试验结束时一次性经口灌胃50%乙醇溶液(14 ml/kg·BW),同时阴性对照组一次性经口灌胃等剂量蒸馏水,在禁食不禁水16 h后处死动物称取肝重,计算脏体比,及时进行肝脏生化指标检测和组织病理学检查。结果受试物中、高剂量组与模型组比较大鼠肝脏中过氧化脂质降解产物丙二醛含量显著降低(P<0.05,P<0.01);受试物低、中剂量组与模型组比较大鼠肝脏中还原型谷胱甘肽含量显著升高(P<0.05,P<0.05)。受试物中、高剂量组与模型组比较脂肪染色评分降低呈现出显著性差异(P<0.05,P<0.01)。结论在本实验条件下,该品牌保肝胶囊对大鼠乙醇致急性肝损伤具有辅助保护作用。

Objective To study the protective effects of a hepatoprotective capsule against alcoholic liver injury by ethanol in rats. Methods The study was conducted in rat models with alcohol-induced liver injury. Three doses groups were given different dosages of the capsule. The negative control group and the model control group were given distilled water. The model control group and three dose groups were given 50% alcohol（ 14 ml/kg. BW）by oral lavage at the end of the experiment,and the negative control group was given the same volume of distilled water. After fasted from feed and water for 16 hours,the rats were weighed and sacrificed,and their livers were isolated and weighed. The organ/weight ratios were calculated, and biochemical and histopathological examinations of livers were timely conducted. Results The contents of MAD in middle-dose and high-dose groups were significantly lower than in the model control group（ P〈0. 05, P〈0. 01）. The contents of GSH were significantly lower in the low-dose and middle-dose groups than in the model control group（ P〈0. 05,P〈0. 05）. The scores of liver adipose tissue stained by HE in both the middle-dose and the high-dose groups were lower than in model control group（ P〈0. 05,P〈0. 01）. Conclusion Under the experimental conditions,the hepatoprotective capsule has an auxiliary protective effect against acute liver injury induced by ethanol in rats.