仿瓷餐具中甲醛的乙酰丙酮分光光度法探究

Determination of Formaldehyde in Imitation Porcelain Tableware by Spectrophotometry with Acetylacetone

目的通过对仿瓷餐具中甲醛的乙酰丙酮分光光度法研究,为食品安全标准制修订提供充分、可靠的数据支持,为食品安全监督管理提供技术支撑。方法利用甲醛在乙酸铵存在下,与乙酰丙酮反应生成黄色的3,5-二乙酰基-1,4-二氢二甲基吡啶化合物,于波长414 nm处比色定量。结果本法标准曲线的回归方程为:y=0.009 77+0.026 0x,r=0.999 9,线性范围(0.20~6.0)mg/L;方法的检出限为0.015 mg/dm^2;定量限为0.045 mg/dm^2;低中高浓度相对标准偏差(RSD)分别为4.42%、4.27%和1.24%;3种仿瓷餐具样品不同加标量回收率分别为勺子95.0%、方碟98.7%、大碗97.6%。结论乙酰丙酮分光光度法能够满足仿瓷餐具中甲醛迁移量的检测要求,操作方便,容易推广,适合于基层特别是市、县疾控中心开展相关的工作。

Objectives To detection the content of formaldehyde in imitation porcelain tableware by acetyl acetone spectrophotometric method to provide full and reliable data for the revision of food safety standard system,and provide technical support for food safety supervision and management. Methods In the excessive presence of ammonium acetate,formaldehyde reacted with acetyl acetone and created a yellow compound generated by formaldehyde and acetylacetone with ammonia,which was detected by spectrophotometry at 414 nm wave length. Results The linear range was（ 0. 20 ~ 6. 0） mg/L. The regression equation was y = 0. 009 77 x ＋ 0. 026 0,and the correlation coefficient was 0. 999 9. The detection limit was 0. 015 mg/dm^2 and the limit of quantitation was0. 045 mg/dm^2. The RSD of low,medium and high concentrations were 4. 42%,4. 27% and 1. 24%,respectively. The recovery of imitation porcelain tableware samples was 90% for spoon,98. 7% for dish and 97. 6% for bowl. Conclusions This method could satisfy the requirement of testing formaldehyde migration in imitation porcelain tableware. The operation is simple and easy to promote and suitable for grass-roots,especially in the city-and county-level Center for Disease Control and Prevention to carry out related works.