3D打印钛金属支架v-PMMA-Ti机械及抗菌性能的体外研究

Construction, mechanical and in vitro antibacterial effects of Ti-6Al-4V porous scaffold combined with vancomycin-loaded PMMA by three-dimensional printing

目的以3D打印支架为基体,构建抗骨感染材料并对其进行优化,制备个性化抗感染的骨内植物材料,探究其结构和组成对抗感染效果的影响。方法利用选择性激光烧结3D打印技术,构建v-PMMA-Ti支架,并测量v-PMMA-Ti支架的组成成分。通过扫描电子显微镜进行支架微结构形态观察。用微孔板高灵敏度分光光度计检测抗生素的释放量。利用琼脂扩散法测定细菌抑菌圈直径。MTT法检测细胞活力和增殖情况。使用Instron 4502单轴测试系统进行支架机械性能测试。结果在v-PMMA-Ti支架中载药抗生素骨水泥可平稳地释放万古霉素,v-PMMA-Ti支架释放速度(2.26±0.19)mg较v-PMMA支架释放速度(0.76±0.21)mg更快,两组相比,差异有统计学意义(P=0.02)。v-PMMA-Ti支架生物力学性能(0.28±0.04)GPa较v-PMMA支架(0.13±0.03)GPa,(P=0.03)更好。同时,v-PMMA-Ti支架有良好的组织相容性,三种支架未见明显的细胞毒性。结论作为药物载体,v-PMMA-Ti支架将万古霉素,骨水泥和金属钛有机结合,与v-PMMA支架相比,抗菌效果更好。此外,其机械性能也相对稳定,v-PMMA-Ti支架有望成为高效、可靠和简便的抗骨感染材料。

Objective To use 3D printed scaffolds as matrix to construct anti-bone-infected materials, prepare personalized anti-infective bone-in-plant materials, and further explore the anti-infection effects. Methods A selective laser sintering 3D printing technology was used to construct an orthogonal microstructure of titanium metal scaffolds. The microstructure of the stent was observed by electron microscopy. A microplate high sensitivity spectrophotometer was used to detect the release of antibiotics. The agar diffusion method was applied to determine the diameter of the bacteria inhibition zone. MTT assay was conducted to detect cell viability and proliferation. The Instron 4502 single-axis test system was used to test the mechanical performance of the stent. Results The drug-loaded antibiotic bone cement in v-PMMA-Ti scaffold released vancomycin steadily, and the v-PMMA-Ti stent release rate（ 2.26 ± 0.19） mg was faster than v-PMMA stent release（ 0.76 ± 0.21） mg with statistically significant differences between the 2 groups（ P = 0.02）. The biomechanical property of v-PMMA-Ti scaffold（ 0.28 ± 0.04） GPa was better than that of the v-PMMA scaffold（ 0.13 ± 0.03） GPa,（ P = 0.03）. At the same time, the v-PMMA-Ti scaffold had good histocompatibility. No obvious cytotoxicity was observed in the 3 scaffolds. Conclusions The 3D printing titanium metal scaffold v-PMMA-Ti organically combines vancomycin, bone cement and titanium metal, with good mechanical and antibacterial property.