经济农作物紫薯保护急性肝损伤小鼠有效部位的研究

Effective extracts of economic crops Ipomoea batatas(L.) Lam for protection of acute liver injury

采用腹腔注射四氯化碳(CCl_4)诱导小鼠急性肝损伤,研究紫薯保肝作用的有效部位。结果发现,紫薯非脂溶性成分及其水提醇沉提取物的低剂量组,均能显著降低小鼠血清中GPT、GOT活力以及肝组织中MDA含量,并显著升高SOD活力(P<0.001);其高剂量组仅降低MDA含量的效果不佳。紫薯乙醇提取物的低剂量组能显著降低GPT、MDA含量以及升高SOD活力(P<0.001),但降低GOT活力效果不佳;其高剂量组对降低GOT和MDA含量效果不佳。紫薯非脂溶性成分和水提醇沉提取物低剂量(400mg/kg bw)时保肝作用较好,且水提醇沉部位是从非脂溶性成分部位提取得到,因此紫薯水提醇沉部位可能是紫薯保肝作用的有效部位。

The acute liver injury in mice was established by intraperitoneal injection carbon tetrachloride to investigate the hepatoprotective effect of I. batatas. The result showed that low dose groups of the nonfat-soluble ingredients and extract by water extraction and alcohol precipitation method, could significantly decrease the activities of GOT and GPT, and the content of MDA, increase the activity of SOD（P〈0.001）, while their high dose groups had not good effect on decreasing MDA content. The low dose group of I. batatas ethanol extract could significantly reduce the activities of GPT, and the content of MDA, increase the activity of SOD（P〈0.001）, while its high dose group couldn＇t significantly decrease the activity of GOT and the content of MDA（P〈0.05）. At the dosage of 400mg/kg bw, the non-fat-soluble ingredients and extract by water extraction and alcohol precipitation had good hepatoprotective effect. And the extract by water extraction and alcohol precipitation came from the non-fat-soluble ingredients, so the extract by water extraction and alcohol precipitation might be the effective extracts of I. batatas for acute liver injury.