婴幼儿米粉中硝酸盐及亚硝酸盐测定方法的改进

Improvement of determination of nitrate and nitrite in infant rice flour

大量实验表明,在采用国标GB 5009.33测定婴幼儿米粉硝酸盐及亚硝酸盐的过程中,由于样品自身的特性,米粉产生糊化,硝酸盐及亚硝酸盐容易被包裹导致样品提取不完全、过滤速度慢、滤液浑浊、回收率低等问题的发生。实验对此进行了方法的改进优化,通过α-淀粉酶水解淀粉后再进行提取,建立了适合婴幼儿米粉中硝酸盐及亚硝酸盐的检测方法。结果表明:方法准确度、精密度均能满足检测方法确认的技术要求,同时也解决了使用国标检测婴幼儿米粉中硝酸盐及亚硝酸盐含量存在的困扰。

A large number of experiments showed that the gelatinization of rice flour easily leaded to nitrate and nitrite incomplete extraction, filtrate turbidity, slow recovery and low incidence in the process of nitrate and nitrite determination in rice flour by GB 5009.33. In this experiment, we improved and optimized the method. After hydrolyzed by α-amylase and extracted, nitrate and nitrite in infant rice noodle was preferable detected and a new method was established. The results showed that measurement accuracy, precision and recovery could meet the technical requirements, and solved the detection problems of nitrate and nitrite in the national standard method for infant rice flour at the same time.