高效液相色谱法测定藏药榜嘎中榜嘎苷A和榜嘎苷C的含量

Determination of the Content of Bangga Flavonoid Glycoside A and Bangga Flavonoid Glycoside C in Tibetan Herb of Bangga by HPLC

目的:建立藏药榜嘎中榜嘎苷A和榜嘎苷C的HPLC含量测定方法。方法:采用Agilent XDB-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-乙腈(9︰1)和0.4%磷酸为流动相,梯度洗脱,流速为1.0 mL·min^(-1),检测波长为325 nm,柱温为30℃。结果:榜嘎苷A进样量在0.1060~6.3621μg线性关系良好(R^2=1.0000),平均回收率为98.7%;榜嘎苷C进样量在0.1059~10.5878μg线性关系良好(R2=0.9995),平均回收率为99.5%。结论:该方法经方法学验证,可用于藏药榜嘎的质量控制。

Objective： To establish an HPLC method for the determination of the content of Bangga flavonoid glycoside A and Bangga flavonoid glycoside C in Tibetan herb of Bangga. Methods： When the chromatogram column was Agilent XDB-C18（4.6 mm×250 mm,5 μm）, the mobile phase consisted of methanol-acetonitrile（9∶1） and 0.4% phosphoric acid with the gradient elution at a flow rate of 1.0 mL·min^（-1）. The detection wavelength was 325 nm and the column temperature was 30 ℃. Results： The calibration curve of Bangga flavonoid glycoside A had a good linear relationship in the sample range of 0.1060-6.3621 μg（R^2=1.0000） and the average recovery rate was 98.7%. The calibration curve of Bangga flavonoid glycoside C also had a good linear relationship in the sample range of 0.1059-10.5878 μg（R2=0.9995） and the average recovery rate was 99.5%. Conclusion： The method was validated and could be used for the quality control of Tibetan herb of Bangga.