着丝粒蛋白A在大鼠心脏的表达及增龄变化

Expression and age-related changes of centromere A in rat heart

目的探讨着丝粒蛋白A(CENP-A)在大鼠心脏中的表达部位及增龄变化。方法取新生1 d、1周龄、2周龄、3周龄、4周龄、3月龄、6月龄大鼠各10只,采用免疫组织化学、免疫荧光、Western blotting、Real-time PCR技术,观察心脏不同部位CENP-A的分布和增龄变化;利用图像分析系统对CENP-A进行定量分析,并对不同年龄组心脏CENP-A含量进行对比。结果大鼠心脏中CENP-A的表达主要分布在血管壁、心外膜及外膜下组织。随着年龄增长血管壁中CENP-A表达基本稳定,心外膜及外膜下组织中CENP-A表达逐渐减少,新生1 d表达最多,3月龄、6月龄明显减少。血管壁中CENP-A主要是在平滑肌细胞表达,且各年龄段表达基本不变。在靠近心外膜的组织中也有少量心肌细胞表达,且随增龄变化而减少。结论大鼠心脏CENP-A的表达随增龄变化逐渐减少,血管壁CENP-A的表达稳定不变。

Objective To study the expression and age-related changes of centromere protein A（ CENP-A） in the rat heart. Methods Ten rats in each group were collected from postnatal 1 day,1 week old,2 weeks old,3 weeks old,4 weeks old,3 months old,6 months old. The expression and age-related changes of CENP-A in the rat heart were observed by the immunohistochemistry,immunofluorescence,Western blotting,and Real-time PCR techniques. The CENP-A proteins of seven groups were quantitated by SPSS 20. 0 statistical software. Results CENP-A was mainly expressed in the vascular wall,epicardium and epicardial tissue and in a very few cardiomyocytes. The CENP-A in vascular wall was of stable expression with age. But the expression of CENP-A in the epicardium and subepicardial tissues was gradually reduced,which showed that postnatal 1 day was the most and 6 months old are the least. CENP-A in the vascular wall was mainly expressed in the smooth muscle cells,and was basically unchanged with ages. A small number of cardiac myocytes near the subepicardium also express CENP-A,and decreased with age. Conclusion The expression of CENP-A in the rat heart gradually decreased with aging,but in the vascular wall remained stable expression.