青海省妊娠高血压综合征患者瘦素基因启动子区G-2548 A单核苷酸的多态性

Polymorphism of leptin gene G-2548 A and pregnancy-induced hypertension syndrome in Han population of Qinghai Province

目的探讨青海省妊娠高血压综合征(PIH)与瘦素基因启动子区单核苷酸多态性的关系。方法选择青海省PIH患者130例,正常妊娠对照组135例,应用聚合酶链反应-限制性内切酶片段长度多态性(PCRRFLP)方法,分析PIH患者和对照组瘦素基因G-2548 A多态性分型并测序验证。结果 PIH组基因型频率GG型、GA型和AA型分别为53.1%、40%和6.9%,对照组分别为60%、34.1%和5.9%。PIH组和对照组瘦素基因G-2548 A位点等位基因A和G频率分布具有差异性(P<0.05),妊娠高血压综合征组G等位基因频率高于对照组(χ2=4.21,P<0.05,OR=0.66,95%CI=0.45~0.99)。结论青海省瘦素基因G2548位点G/A基因多态性与其妊娠高血压的易感性有关。G等位基因可能为妊娠高血压综合征的易感基因,A等位基因可能为妊娠高血压综合征的保护基因。

Objective To investigate the relationship between polymorphism of leptin gene promoter region G-2548 A and pregnancy-induced hypertension syndrome（ PIH） in Han population of Qinghai Province. Methods A total of 130 PIH subjects and 135 normal pregnancy subjects were studied. The genotype of leptin was analyzed by polymerase chain reaction fragment length polymorphism（ PCR-RFLP）. The mutation was confirmed by sequencing. Results The frequencies of leptin GG,GA,and AA genotype in the PIH group were 53. 1%,40% and 6. 9%,and in the control group were 60%,34. 1% and 5. 9% respectively. There was difference in frequency distribution of leptin genotype between the PIH and control groups. The frequency of G gene in the PIH group was higher than the control group（ χ2= 4. 21,P 0. 05,OR = 0. 66,95% CI = 0. 45-0. 98）. Conclusion Leptin gene G-2548 A is significantly correlated with PIH in Han group of Qing Hai. G allele may be a susceptible gene to PIH（ OR = 1. 361,95% CI： 1. 01-1. 83） and A allele may be a protective gene to PIH（ OR = 0. 901,95% CI： 0. 82-1. 00）.