百合固金汤加减对柯萨奇病毒16型肺损伤肺表面活性物质及炎症指标的影响

The Influence of Modified Lily Bulb Metal-Securing Decoction on Pulmonary Surfactant and Inflammatory Markers in Coxsackievirus 16 Lung Injury

目的:观察感染柯萨奇病毒16(coxsackievirus,CA16)的恒河幼猴肺表面活性物质及炎症反应变化,探讨百合固金汤加减在CA16防治中的价值。方法:34只SPF级恒河幼猴,按照随机数字表法分为治疗组17只和对照组17只,雌雄兼用,采用喷雾攻毒方式,即模拟正常情况下呼吸道感染方式,接种滴度为6.95 g CCID50·m L^(-1)的病毒液1 m L,制造重症手足口病肺损伤的模型,造模成功后对照组不进行治疗,治疗组以百合固金汤加减灌肠,灌肠量:每次20~30 m L,每天灌肠1次,连续记录24 h、72 h、120 h、168 h肺表面活性物质、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-8(interleukin-8,IL-8)的表达结果。结果:两组恒河幼猴肺支气管肺泡灌洗液中二棕榈酸磷脂酰胆碱(two palmitic acid phosphatidyl choline,DPPC)/总磷脂(total phosphatide,PL)及SP-C蛋白mRNA同期比较,治疗组DPPC/PL比值及SP-C蛋白mRNA的表达明显高于对照组(P<0.05);治疗组恒河幼猴IL-6及IL-8凋亡较多,与对照组比较有统计学意义(P<0.05)。结论:百合固金汤可对手足口病肺损伤起到防治作用。

Objective： To observe the changes of pulmonary surfactant and inflammatory response in Ganges RIver monkeys infected with CA16,and to explore the value of Modified Lily Bulb Metal-Securing Decoction in the prevention and treatment of CA16.Methods： Thirty-four SPF Ganges RIver monkeys were randomly divided into the treatment group with 17 cases and the control group with 17 males and females. After the success of establishing the models,the control group was not treated. The treatment group was treated with Modified Lily Bulb Metal-Securing Decoction for gavage. The amount was between 20 and 30 m L,once a day. And 24 hours,72 hours,120 hours and 168 hours were continuously recorded to observe the expression of pulmonary surfactant（ DPPC/PL and SP-C protein mRNA）,and interleukin-6（ IL-6） and interleukin-8（ IL-8） expression. Results： The related indexes of the treatment group were significantly changed compared with those of the control group. Conclusion： Application of Chinese medicine can prevent and treat lung injury caused by hand foot mouth disease.