摘要
为了研究SibZIP8介导谷子抗旱的分子机制,试验根据SibZIP8 cDNA序列设计引物,以晋谷21的cDNA为模板,通过PCR扩增SibZIP8基因的全长cDNA。将所得的cDNA序列与表达载体p CAMBIA1302连接。通过对阳性克隆进行测序筛选,确定pCAMBIA1302-SibZIP8超量表达载体构建成功。该超量表达载体可用于SibZIP8基因功能的研究。
To find the molecular mechanism of Setaria. italica SibZIP8 responded to drought,primers were designed according to SibZIP8 cDNA sequence. SibZIP8 cDNA was amplified from Jingu 21 cDNA. The total CDS sequence of SibZIP8 was cloned into the expression vector pCAMBIA1302. The construction of p CAMBIA1302-SibZIP8 was identified by sequencing the positive clones. This overexpression vector can use to research the function of SibZIP8.
作者
成亮
刘盼
邢恒荣
张莉
CHENG Liang;LIU Pan;XING Hengrong;ZHANG Li(Shanxi Pharmaceutical Vocational College,Taiyuan 030031,China;College of Agronomy,Shanxi Agricultural University,Taigu 030801,China)
出处
《山西农业科学》
2018年第8期1235-1238,共4页
Journal of Shanxi Agricultural Sciences
基金
国家自然科学基金项目(31501323)
山西省回国留学人员科研资助项目(2016-071)
山西农业大学专业提升计划(TSJH1406)
关键词
谷子
bZIP转录因子
超量表达载体
基因功能
foxtail millet
bZIP transcription factors
overexpression vectors
gene function
