摘要
[Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [Methods]With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer,polyploid induction was performed twice on diploid plants by addition method,and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results]Among the 7 polyploidy plants,four materials were tetraploid,the concentration and treatment time of which were 300 mg/L and 7 and 14 d,respectively; and three materials were octaploid,the concentrations and treatment time of which were 300 and 500 mg/L and 14 d,respectively. [Conclusions]The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects,and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore,colchicine is an polyploid inducer suitable for L. edulis.
[ Objectives] This study was conducted to provide high-quality polyploid for returning the grain plots to forestry and promote the rapid development of Lonicera edulis Turcz. [ Methods] With superior individuals of wild L. edulis in Changbai Mountains as experimental materials and colchicine as inducer, polyploid induction was performed twice on diploid plants by addition method, and 7 polyploidy plants were identified from 11 regenerated plantlets. [Results] Among the 7 polyploidy plants, four materials were tetraploid, the concentration and treatment time of which were 300 mg/L and 7 and 14 d, respectively; and three materials were octaploid, the concentrations and treatment time of which were 300 and 500 mg/L and 14 d, respectively. [ Conclusions] The results of this study showed that colchicine had a higher polyploid induction rate for L. edulis with lower toxic and side effects, and the polyploidy plants could restore to normal growth state after several times of cubculture. Therefore, colchicine is an polyploid inducer suitable for L. edulis.
基金
Supported by The Cooperative Project between the Yanbian University,China
National Institute of Horticultural and Herbal Science of the Rural Development Administration(RDA)of the Republic of Korea
