摘要
This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats were divided into two groups, one of which was normal control and the other was fed with fructose-rich diet. The mRNA was isolated and purified from the skeletal muscle of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microarray. The microarray was scanned, analyzed and repeated for two times. Among the total 4 096 tested genes, 140 genes were differently expressed, 62 up-regulated,78 down-regulated, the expression of Ptprd and Gilz and multiple genes of oxidative metabolism is associated in insulin resistance. The differential expression of gene may be related to the pathogenesis of insulin resistance.
This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats were divided into two groups, one of which was normal control and the other was fed with fructose-rich diet. The mRNA was isolated and purified from the skeletal muscle of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microarray. The microarray was scanned, analyzed and repeated for two times. Among the total 4 096 tested genes, 140 genes were differently expressed, 62 up-regulated,78 down-regulated, the expression of Ptprd and Gilz and multiple genes of oxidative metabolism is associated in insulin resistance. The differential expression of gene may be related to the pathogenesis of insulin resistance.
基金
Supported by"125"Major Scientific and Technological Project of Education Department of Guizhou Province[QJHZDZXZ(2012)013]
Guiyang Science and Technology Bureau Project[2012204]
