隐甲藻藻渣醇水提取物的抗氧化活性研究

Antioxidant study of different polarity fractions of alcohol aqueous extracts of Crypthecodinium cohnii residue

本研究采用总抗氧化能力、DPPH自由基清除能力、亚铁离子螯合能力及总还原力等抗氧化指标评价隐甲藻(Crypthecodinium cohnii)藻渣醇水提取物不同极性萃取物的抗氧化能力,采用分光光度法测定样品中总酚和总黄酮含量。结果表明隐甲藻渣不同极性萃取物均具有一定的抗氧化能力,其中乙酸乙酯相和石油醚相总抗氧化能力和亚铁离子螯合能力较高,水相和正丁醇相的DPPH自由基清除能力和总还原能力较高,且在一定质量浓度范围内存在量效关系。相关性分析结果显示,不同极性萃取物的总多酚含量与DPPH自由基清除能力和总还原能力显著正相关,而总黄酮仅与总抗氧化能力和金属离子螯合能力呈正相关关系,提示除酚类和黄酮类化合物外,可能还含有其他抗氧化成分。本研究表明隐甲藻藻渣具有较好地抗氧化活性,有待合理开发利用,其可能存在的其他抗氧化活性成分也有待于进一步明确。

Total antioxidant capacity（TAC）,DPPH radical scavenging activity（DPPH）,ferrous ion-chelating activity（FICA）,reducing power（RP）were measured to evaluate the antioxidant capacity of different polarity fractions of alcohol aqueous extracts of Crypthecodinium cohnii residue,aiming to provide theoretical reference value for comprehensively utilizing C.cohnii.Folin-Ciocalteu and spectrophotometry method was used to determine total phenolic content（TPC）and total flavonoid content（TFC）.The results showed that different polar fractions of the residue of C.cohnii had certain antioxidant capacity with a mass concentration-depended effect.The fraction of ethyl acetate and petroleum ether showed higher TAC and FICA than that of water and n-butanol,which showed higher DPPH and RP.Correlation analysis indicated that TPC was positively correlated with DPPH and RP,while TFC was positively with TAC and FICA.The residue of C.cohnii exhibits good antioxidant activity,while its effective active ingredients need to be further studied.