南极罗斯海区域可培养微生物分离鉴定及产低温酶能力初步筛选

Diversity of culturable bacteria and fungi isolated from Ross Sea region of Antarctica separation,identification and initial screening for the ability to produce low-temperature enzymes

为了利用南极微生物资源、探索南极罗斯海区域可培养微生物多样性,利用传统平板培养法对中国第33次南极科学考察采自南极罗斯海6个站位的海洋沉积物样品进行了细菌、真菌的分离培养。经细菌16SrRNA、真菌ITS基因序列检测及系统发育分析,共获得5个属的36株细菌和6个属的29株真菌。其中嗜冷杆菌属为优势细菌类群,枝孢属为优势真菌类群。该结果表明南极罗斯海区域具有丰富的微生物多样性。细菌API 20NE生理生化及真菌产胞外酶活性实验显示,分离得到的细菌和真菌绝大多数都有低温酶活性。研究结果为南极罗斯海区域可培养微生物的多样性认识和低温酶产生菌资源获取与开发利用提供了支撑。

To explore the cultivable microbial diversity in the Ross Sea region of Antarctica and take advantage of Antarctic recourses,we isolated bacteria and fungi from the samples of 6 stations in Ross Sea during the 33 th China Antarctic scientific expedition.16 SrRNA and fungal ITS analysis suggest that a total of36 strains of bacteria are from 5 genera and 29 fungi are from 6 genera.Among them,the Psychrobacteris the dominant bacteria,Cladosporiumis the dominant fungi.The results show that the microorganisms in the sediments of Ross Sea are rich in diversity.20 NE API test and fungi extracellular enzyme activity test showed that most of the isolated bacteria and fungi can produce different low-temperature enzymes.The results provide support for the diversity of culturable microorganisms in the Ross Sea area of Antarctica and the acquisition and development of low-temperature enzyme-producing bacteria.