3D打印制备HA/PCL复合材料组织工程支架的研究

Preparation of hydroxyapatite/polycaprolactone (HA/PCL)composite tissue engineering scaffolds by 3D printing

应用熔融沉积成型技术(FDM)制备羟基磷灰石(HA)/聚己内酯(PCL)组织工程支架,探讨其内部结构和力学性能。以羟基磷灰石和聚己内酯为原料,采用熔融共混技术分别制备HA质量分数为20%的nano-HA/PCL和micro-HA/PCL复合材料,使用自主研发的熔体微分FDM 3D打印机制备HA/PCL复合材料组织工程支架。通过显微镜观察发现,所制备的nano-HA/PCL和micro-HA/PCL组织工程支架具有均匀分布且相互连通的近似矩形的孔隙。nano-HA/PCL和micro-HA/PCL组织工程支架的断面图分析结果表明,nano-HA/PCL组织工程支架中HA粒子分布均匀,而micro-HA/PCL组织工程支架中HA粒子发生了团聚,导致nano-HA/PCL组织工程支架的拉伸强度和弯曲强度均高于micro-HA/PCL组织工程支架。因此,利用熔体微分FDM 3D打印机打印生物活性nano-HA/PCL复合材料组织工程支架在骨组织工程中具有潜在的应用前景。

Hydroxyapatite/polycaprolactone（ HA/PCL） scaffolds have been prepared by a fused deposition modeling（ FDM） technique using hydroxyapatite（ HA） and polycaprolactone（ PCL） as raw materials and their internal structure and mechanical properties were explored in detail. Nano-HA/PCL and micro-HA/PCL composites with20 wt. % HA were prepared by melt blending technology and,furthermore,HA/PCL composite tissue engineering scaffolds were prepared using a self-developed melt differential FDM 3 D printer. By microscopic observations,it was found that the prepared nano-HA/PCL and micro-HA/PCL tissue engineering scaffolds have uniformly distributed and interconnected nearly rectangular pores. By observing the cross-sectional view of the nano-HA/PCL scaffold and the micro-HA/PCL scaffold,it can be seen that the HA particles in the nano-HA/PCL scaffold are evenly distributed and the HA particles in the micro-HA/PCL scaffold are agglomerated,which contributes to the nano-HA/PCL scaffolds having higher tensile strength and flexural strength than the micro-HA/PCL scaffolds. Therefore,the bio-active nano-HA/PCL composite scaffolds prepared using melt differential FDM 3 D printers have better potential application prospects in bone tissue engineering.