常温稀释液不同温度保存蓝狐精液的效果研究

Study on the effect of preserving blue fox semen with room temperature diluent at different temperatures

为了研究常温稀释液不同温度保存蓝狐精液的效果,试验采用东林Ⅱ号狐常温精液稀释液对8只引进芬兰种蓝狐的精液稀释后,分别在36℃（体温）、25℃（常温）、4℃（低温）条件下对精子的存活时间进行检测。结果表明：4℃条件下,有效生存指数平均值为17.21;25℃条件下,有效生存指数平均值为6.19;36℃条件下,有效生存指数平均值为3.94。36℃精子保存时间不宜超过3 h,25℃精子保存时间不宜超过5 h,4℃精子保存时间不宜超过20.5 h。

The aim of the present study was to investigate the effect of preserving blue fox semen with room temperature diluent at different temperature. The sperm survival time of the eight imported Finnish blue foxes was determined at 36 ℃（ body temperature）,25 ℃（ room temperature） and 4 ℃（ low temperature） by using the room temperature semen dilution solution of Donglin Ⅱ fox. The results showed that the effective survival index was 17. 21 at 4 ℃. When the temperature reached 25 ℃,the effective survival index was 6. 19. When the temperature reached36 ℃,the effective survival index was only 3. 94. Therefore,it was suggested that the sperm preservation time should not exceed 3 hours at36 ℃,and 5 h at 25 ℃,and not more than 20. 5 h at 4 ℃.