TNF-α启动子区单核苷酸多态性与中国维吾尔族白癜风的相关性

Association of single nucleotide polymorphism of TNF-α and vitiligo in the patients of Uygur nationality in China

目的:明确TNF-α启动子区基因多态性与中国维吾尔族白癜风发病机制的相关性。方法:PCR扩增316例维吾尔族白癜风患者和330例健康对照组外周血目的片段DNA,DNA全自动测序仪对TNF-α启动子区基因rs1799964、rs1800630、rs1799724三个位点单核苷酸基因多态性进行测序。结果:白癜风患者TNF-αrs1799964、rs1800630、rs1799724基因型和等位基因频率与对照组比较均无统计学意义(P>0.05)。TNF-αrs1799724 T等位基因频率在白癜风、有无家族史间比较,差异有统计学意义(均P<0.05)。结论:TNF-α启动子区rs1799724位点单核苷酸多态性可能参与中国维吾尔族白癜风的发生和发展。

Objective： To determine the association of TNF-α single nucleotide polymorphisms（ SNP）and vitiligo in the patients of Uygur nationality in China. Methods： The aim gene fragment extracted from the peripheral blood DNA of 316 uygur patients with vitiligo and 330 uygur healthy controls. Three loci polymorphisms rs1799964,rs1800630,rs1799724 in TNF-α promoter were detected by the sequencing method. Results： There was no significant differences in the frequency of rs1799964,rs1800630 and rs1799724 between the patients and controls（ P〉0.05）. There were significant differences in the rs1799724 T allele frequency between the patients with and without family history（ Ps〈0.05）. Conclusion： TNF-α promoter rs1799724 SNP may be associated with vitiligo in Chinese uygur.