茶多酚二元醇脂质体的制备及质量评价

Preparation and quality evaluation of tea polyphenol binary ethosomes

目的:制备茶多酚二元醇脂质体(TP-EL)并对其质量进行评价。方法:以包封率作为评价指标进行正交试验筛选最佳处方及制备工艺,并评价二元醇脂质体的质量。结果:各因素最佳的水平组合为:磷脂浓度为4%、磷脂与胆固醇的比为5∶1、药脂比为1∶8、搅拌时间为40 min。所制备的脂质体大多数为单室脂质体,平均粒径为123.0±13.2 nm,多分散性指数(PDI)为0.15±0.07。TP-EL的Zeta电位为-35.2 mV,且包封率达71.82±2.03%。结论:该制剂制备简便、质量稳定。

Objective： To prepare tea polyphenol binary ethosomes（ TP-EL） and evaluate its quality.Methods： Encapsulation efficiency was taken as evaluation index to optimize the formula and preparation technology of TP-EL. The quality of TP-EL was assessed. Results： The optimal formula and preparation technology were as follows： the concentration of phosppholipids was 4%,the ratio of phospholipids/cholesterol was 5∶1,the ratio of TP/phospholipids was 1 ∶8 and the stirring time was 40 min. The results showed that most of the TP-EL was unilamellar structure. The TP-EL had an average size of 123.0±13.2 nm with polymer dispersity index（ PDI） of 0.15±0.07. The surface charge of TP-EL was-35.2 mV. Moreover,TP was readily incorporated into ethosomes with encapsulation efficiency of 71.82± 2.03%. Conclusion： The preparation technology for TP-EL was simple and stable.