大学生口腔齿龈内阿米巴感染情况调查

Survey of the infection of Entamoeba gingivalis in college students

[目的]调查大学生齿龈内阿米巴的感染情况.[方法]选择165名大学生进行问卷调查,内容包括性别、口腔疾病、刷牙习惯、漱口情况、咀嚼口香糖、吸烟及近1个月使用抗生素情况等.采用一次性棉签从牙周组织或齿龈隙取垢物或渗出液进行涂片,行吉姆萨染色,并在显微镜下进行观察.[结果]受检者齿龈内阿米巴感染率为73.94%,其中男生和女生感染率分别为77.42%和71.84%.有口腔疾病者齿龈内阿米巴感染率为81.93%,明显高于无口腔疾病者的65.85%(P<0.05);每日刷牙1次和2次者齿龈内阿米巴感染率分别为85.29%和67.94%,差异有统计学意义(P<0.05);经常嚼口香糖者齿龈内阿米巴感染率为51.61%,显著低于不经常嚼口香糖者的75.22%(P<0.05);近1个月内使用抗生素者齿龈内阿米巴感染率为95.24%,明显高于未使用者的70.83%(P<0.05);吸烟者与不吸烟者齿龈内阿米巴感染率分别为92.86%和66.92%,差异有统计学意义(P<0.05).齿龈内阿米巴感染率在不同性别、刷牙前后及漱口习惯间差异均无统计学意义(P>0.05).[结论]大学生口腔齿龈内阿米巴感染率较高,可能原因认为与口腔疾病和生活习惯相关.

OBJECTIVE To investigate the infection status of Entamoeba gingivalis in college students.METHODS165 undergraduates were selected for the questionnaire survey,including gender,oral diseases,brushing habits,gargling situation,chewing gum,smoking and the situation of antibiotics use for nearly1 month and so on.Disposable cotton swabs were used to remove scale or exudate from periodontal tissue or gum gap for smear,and it was observed under optical microscope after Giemsa staining.RESULTS The infection rate of Entamoeba gingivalis was 73.94% in all subjects,77.42% and 71.84% in males and females respectively.The infection rate of the undergraduates with oral diseases（81.93%）was significantly higher than that of the undergraduates without oral diseases（65.85%,P〈0.05）.The infection rates of the undergraduates who brush teeth once a day and twice a day were 85.29% and 67.94% respectively,the difference was statistically significant（P〈0.05）.The infection rate of the undergraduates who usually chew gum（51.61%）was lower than that of the undergraduates who do not（75.22%,P〈0.05）.The infection rate of the undergraduates who use antibiotic recently（95.24%）was higher than that of the undergraduates who do not use it（70.83%,P 〈0.05）.The infection rate of the smokers and non-smokers was 92.86% and 66.92% respectively,the difference was statistically significant（P〈0.05）.No significant difference was found in gender,before or after brushing teeth and gargling habits（P〉0.05）.CONCLUSION The infection rate of Entamoeba gingivalis was high in undergraduates,which might be related to oral diseases and living habits.