PDGFRα在成人胶质母细胞瘤患者中的表达及临床意义

Expression and clinical significance of PDGFRα in Chinese adult glioblastoma

目的探讨血小板源性生长因子受体α(PDGFRα)在成人胶质母细胞瘤(GBM)患者中的表达并评价其临床意义。方法选取2013年7月至2016年10月间中国医学科学院肿瘤医院和安徽医科大学第二附属医院收治的278例成人GBM患者,采用组织芯片-免疫组化染色技术检测278例患者瘤脑组织及10例非瘤脑组织中PDGFRα表达情况,10例非瘤脑组织为切除深部胶质瘤而造瘘获得的表浅正常组织,并对PDGFRα阳性表达与GBM临床病理参数和预后关系进行分析。结果 PDGFRα在10例非瘤脑组织中均未见明显阳性信号,而在278例GBM标本中41例为强阳性,阳性率为14.7%,主要定位在细胞浆和细胞膜。PDGFRα表达与p53表达显著正相关,差异有统计学意义(P<0.01),PDGFRα表达与性别、年龄、卡氏生活治疗评分(KPS)、病理类型、治疗方式及IDH1状态等临床病理参数均无相关性,差异无统计学意义(P>0.05)。多因素Cox分析结果表明KPS、术后治疗方式及PDGFRα阳性表达是GBM的独立预后因素,差异均有统计学意义(均P<0.01)。结论 PDGFRα在成人GBM患者中存在异常表达,可作为GBM分子分型、辅助治疗及预后评估的标志物。

Objective To detect the expression of platelet-derived growth factor receptor α（ PDGFRα） in Chinese adult glioblastoma（ GBM） and evaluate its clinical significance. Methods Two hundred and seventy-eight Chinese adult GBM treated at The Second Hospital of Anhui Medical University and Cancer Hospital,Chinese Academy of Medical Sciences were selected from July 2013 to October 2016.The expression of PDGFRα in brain tissues was detected among 278 Chinese adult GBM and 10 individuals who obtained normal superficial tissues after resection of deep glioma and then stoma using tissue microarrayimmunohistochemistry staining. The relationship between PDGFRα expression and clinicopathological parameters and prognosis was analyzed. Results Obvious positive signals were observed in PDGFRα in the10 matched individuals but its expression is strongly high in the 41 GBM specimens with the positive rate of14. 7% and mainly located in the cytoplasm and cell membrane. The expression of PDGFRα was positively correlated with the expression of p53（ P〈0. 01）,but there was no statistically significant correlation with clinicopathological parameters such as gender,age,Karnofsky performance status（ KPS）,pathological type,treatment regimens and IDH1 status（ P〈0. 05）. The results of log-rank analysis showed that the positive expression of PDGFRα was negatively associated with the survival time（ P = 0. 05）. Multivariate Cox analysis revealed that KPS,postoperative treatment regimens and positive expression of PDGFRα were independent prognostic factors for GBM（ all P〈0. 01）. Conclusion Unusual expression of PDGFRα in Chinese adult GBM may be served as a marker for the prediction of GBM molecular subtype,adjuvant therapy and prognosis evaluation.