黄连上清片中黄芩的薄层鉴别与黄芩苷的含量测定

Identification and Determination of Baicalin in Huanglian Shangqing Tablets

目的提高黄连上清片的质量标准。方法研究采用薄层色谱(TLC)法鉴别片中的黄芩,同时采用HPLC法测定片中黄芩苷的含量。结果 TLC结果表明片中可以检出黄芩,黄芩苷在0.12~2.21μg(r=0.9999),范围内线性关系良好,平均加样回收率为99.94%,RSD为0.47%(n=6)。结论该方法简便,准确,专属性强,可用于黄连上清片的质量控制。

OBJECTIVE To improve the quality standards Huanglianshangqing Tablets. METHODS TLC and high performance liquid chromatography （HPLC） were employed to identification and determination of baicalin in Huanglian Shangqing Tablets. RESULTS TLC result showed that baicalin can be detected in the Tablets. The line- ar range of baicalin was 0. 12 -2. 2μg （r =0. 9999） and the average recovery rate was 99. 94% ,RSD was 0. 47% （n = 6）. CONCLUSION The method is simple, accurate, specific and can more effectively control the quality of Huanglianshangqing Tablets.