HPLC法同时测定复方蒲芩胶囊中4种有效成分的含量

Simultaneous Determination of Four Active Ingredients in Fufang Puqin Capsule by HPLC

目的建立同时测定复方蒲芩胶囊中绿原酸、咖啡酸、黄芩苷和盐酸小檗碱4种成分含量的方法。方法采用高效液相色谱法,色谱柱为Shim-pack VP-ODS,流动相为乙腈-0.02mol·L^(-1)磷酸溶液(梯度洗脱),流速为1.0mL·min^(-1),检测波长为280nm,柱温为30℃,进样量为20μL。结果绿原酸、咖啡酸、黄芩苷和盐酸小檗碱的线性范围分别为0.004~0.125、0.004~0.126、0.035~1.058、0.135~4.050mg·mL^(-1)(r分别为0.9996、1.000、0.9995、0.9997);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为99.2^(-1)02.7%、100.4~103.1%、100.3~102.9%、99.6~102.2,RSD分别为1.08%、0.92%、0.76%、0.88%(n=9)。结论该方法简单快速、准确性好,可用于复方蒲芩胶囊的质量控制。

OBJECTIVE To establish a method for simultaneous determination of chlorogenic acid, caffeic acid, baicalin,berberine hydrochloride in Fufang Puqin Capsules. METHODS HPLC was performed on the column of Shim-pack VP-ODS with mobile phase of nitrile-0. 02mol · L-1 phosphoric acid solution（ gradient elution） at a flow rate of 1.0mL · min- 1, the detection wavelength was 280nm, the column temperature was 30℃, and the injection vol- ume was 20μL. RESULTS The linear range was 0. 004 - 0. 125mg · mL-1 for chlorogenic acid （ r = 0. 9996 ） , T 0. 004 - 0. 126mg · mL- 1 for caffeic acid （ r = 1. 0000 ）, 0. 035 - 1. 058rag - mL- 1 for baicalin （ r = 0. 9995 ） and 0. 135 - 4. 050mg · mL -1 for berberine hydrochloride （ r = 0. 9997 ） ; RSDs of precision, stability and reproducibility tests were lower than 2. 0% , reconeries were 99. 2 - 102. 7% （ RSD = 1.08%, n = 9）, 100. 4-103.1% （ RSD = 0. 92% ,n = 9 ）, 100. 3 - 102. 9% （ RSD = 0. 76%, n = 9 ）, 99. 6 - 102.2 （ RSD = 00. 88%, n = 9 ）, respectively. CONCLUSION The method is Simple, rapid and reproducible, and canbe used for the quality control of Fufang PuQin capsule.