HPLC法测定化妆品中12种邻苯二甲酸酯类化合物

Determination of 12 kinds of phthalates in cosmetics by HPLC

采用亚3μm填料的Agilent Poroshell 120 EC-C_(18)色谱柱(100 mm×4.6 mm,2.7μm)对化妆品中的12种邻苯二甲酸酯类化合物进行分析,以甲醇-乙腈-水为流动相,梯度洗脱,检测波长230 nm。结果表明,12种成分在10 min内完成分离,在5.0~100 mg/L范围内线性关系良好。方法检出限为1.0~40.0 mg/kg,定量下限为3.5~150.0 mg/kg,平均加样回收率在89.4%~107.1%之间,RSD在0.3%~1.5%之间。

The chromatographic separation of 12 kinds of phthalates in cosmetics was achieved on an Agilent Poroshell 120 EC-C18（100 mm × 4.6 mm,2.7 μm） filled with particles of sub-3 μm,with the mobile phase of methanol-acetonitrile-water by gradient elution,at the detection wavelength of 230 nm.The results indicate that 12 components are completely separated in 10 min,and the linear relationship is good in the range of 5.0-100 mg/L.The detection limits range from 1.0 mg/kg to 40.0 mg/kg,and the lower limits of quantitation range from 3.5 mg/kg to 150.0 mg/kg.The average recoveries are in the range of 89.4%-107.1%,and RSDs are in the range of 0.3%-1.5%.