应用组学技术分析伪结核棒状杆菌的致病性

Application of omics technology on Corynebacterium pseudotuberculosis pathogenicity analysis

胞内寄生病原伪结核棒状杆菌(Corynebacterium pseudotuberculosis,Cp)不仅对全球养殖业造成巨大的经济损失,且可感染人而危害公共卫生安全。组学技术的快速发展促进了Cp致病性的研究。通过基因组测序分析获得72株Cp全基因组序列,初步明确了羊型Cp与马型Cp的结构、进化关系及致病性差异的可能原因;基于蛋白质组学研究,发现了磷脂酶D(PLD)、丝氨酸蛋白酶(CP40)及神经氨酸酶H(NanH)等与Cp生理学、毒性和免疫相关及其他未知功能的蛋白;利用转录组学发现Cp在高渗透压、热休克或酸性条件等恶劣环境中以粘附、应激和氧化还原反应基因表达差异最为典型。本文在介绍Cp基因组测序情况基础上结合作者获得的Cp宣汉株(XH02)基因组序列进行比较基因组学和SNP进化分析,同时对蛋白质组学和转录组学技术在Cp致病机制研究中的应用概况进行综述。

Corynebacterium pseudotuberculosis is an interesting bacterium due to its great zoonotic potential and because it causes considerable economic losses worldwide. The rapid development of omics technology has promoted the study of C. pseudotuberculosis pathogenicity. A total of 72 C. pseudotuberculosis genome sequences were obtained by genome sequencing analysis, and the structure, evolution and pathogenicity differences of C. pseudotuberculosis biovar equi or biovar ovis were identified. Based on the proteomics study, proteins such as phospholipase D（PLD）, serine protease（CP40） and neuraminidase H（Nan H） were found to be related toC. pseudotuberculosis physiology, toxicity and immunity, and other unknown functions. With the transcriptomics, it was found that defense against oxidative stress, adhesion and regulation were most typical in the gene expression difference in harsh environments such as high osmotic pressure, heat shock or acidic conditions. In this paper, we analyzed the C. pseudotuberculosis Xuanhan strain（XH02） we obtained with comparative genomic and SNP evolution, and the application of proteomics and transcriptomics in C. pseudotuberculosis pathogenesis was reviewed.