复合Ι型胶原海绵创伤止血的动物实验研究

Study on haemostatic property of compound collagen I sponge

目的 探讨猪脚肌腱提取的Ι型胶原与壳聚糖混合 ,交联冻干后形成的复合Ι型胶原海绵对创伤创面出血的止血效果。方法 用新西兰大白兔作实验动物 ,对其皮下出血、体表浅动静脉断裂损伤、肝损伤进行止血试验 ,同时与明胶海绵、纱布等止血效果作比较 ,观察其止血时间、敷料与创面的粘合等情况 ,并定期观察创面愈合和体内吸收情况。结果 本试验表明 :复合Ι型胶原海绵的止血时间约 2分钟 ,明胶海绵为 3.5分钟 ,纱布压敷为 10分钟以上 ;复合Ι型胶原海绵和明胶海绵均能在体内降解吸收。结论 复合Ι型胶原海绵止血效果最好 ,无论是止血时间、止血效果、与伤口的粘附能力都优于其他 2种材料 ,并能在体内降解吸收 ,因此 ,是一种较理想的创伤止血材料。

Objective To detect haemostatic property of a new compound material made of chitosan and collagen I from porcine tendon.?Methods The model of bleeding trauma was made by 1cm×1cm wound on the ear of New Zealand Rabbit including to cutting off an ear artery,and by resection of 1cm×1cm block on the liver, patching the wound with sponge we made, gelatin sponge and gauze,the haemostatic effect and time of degradation of sporige were recorded.?Results The haemostatic time of chitosan collagen I sponge was 2 min, and gelatin sponge was 3.5 min, and gauze was 10 min. Both the chitosan collagen I and gelatin sponge could be degraded in vivo.?Conclusion The haemostatic result of chitosan collagen I sponge is better than that of gelatin sponge and gauze . The chitosan collagen I sponge can also be degraded in vivo. So, it is an ideal haemostatic material.