马兜铃酸对斑马鱼肾损伤因子(KIM-1)表达的影响

Effect of Aristolochic Acid on Expression of KIM-1 in Zebrafish

采用分子定量方法检测斑马鱼(Danio rerio)幼鱼组织中KIM-1蛋白含量和基因表达水平,了解马兜铃酸处理后斑马鱼体内肾损伤因子的表达变化情况,并与幼鱼表型变化相比较,探讨KIM-1作为肾功能特异标志物在斑马鱼肾功能损伤评价中的应用价值。结果显示,马兜铃酸处理30 h后,幼鱼出现水肿,水肿发生率呈剂量依赖性特点。0.5~5.0μmol/L马兜铃酸处理组幼鱼水肿发生率与对照组差异不明显,但幼鱼KIM-1因子的蛋白含量及Kim-1基因表达水平比正常对照组显著升高,在马兜铃酸浓度为2μmol/L时达到最高。表明在肾功能损伤检测中,KIM-1是一种比表型更早发生变化的指标。

To explore the application possibility of kidney injury molecule-1（KIM-1） as a specific biomarker in the evaluation of the renal function,we studied the changes of kidney injury molecule-1 content and its m RNA level in Zebrafish（Danio rerio） after aristolochic acid treatment.The results were compared with the phenotypic changes of Zebrafish.The larvae showed periocular and pericardial edema in a dose-response manner after exposure to aristolochic acid for 30 hours（Table 1）.Although the incidence of edema in 0.5-5.0 μmol/L aristolochic acid treated groups had no significant difference compared with the control,the protein content and m RNA expression level of KIM-1 in larvae tissue were significantly higher than the control（Table 1,Fig.1）.The peak appeared in the 2 μmol/L aristolochic acid treated group（Fig.2,3）.From the result we can conclude that KIM-1 is a more sensitive indicator for detection of renal injury,whose changes are earlier than the morphological changes in zebrafish.