摘要
本文通过马来酰化胶原(Col-MA),巯基化透明质酸(HA-SH)和聚乙二醇二丙烯酸酯(PEGDA)在生理条件下的迈克尔加成反应制备了新型胶原基原位水凝胶。圆二色谱图证明Col-MA仍然具有完整的三螺旋结构。流变学分析结果表明控制各组分含量可以使水凝胶成胶时间由49.75分钟缩短到18秒,储能模量由30.47 Pa增加到6582.24 Pa。水凝胶能快速溶胀至平衡,含水量达到90%以上。细胞培养实验结果显示水凝胶具有良好的生物相容性。基于这些优点,这种原位水凝胶在组织工程领域有较好的应用前景。
In this work, novel collagen-based in-situ hydrogels were prepared through Michael addition reaction ofmaleic acylated collagen （CoI-MA）, thiolated hyaluronan （HA-SH） and PEGDA. Circular dichroism （CD） spectras revealed the complete triple helix structure of CoI-MA. Rheology analysis indicated that the gelation time decreased from 49.75 rain to 18 s and the storage modulus increased from 30.47 Pa to 6582.24 Pa by controlling the content of each component. The hydrogels swelled to equilibrium quickly and the water contents were above 90 %. Furthermore, the hydrogels showed good biocompatibility. Taken together, these in-situ hydrogels show strong potential in tissue engineering.
作者
郑路
张慧杰
Zheng Lu, Zhang Huijie(Pharmaceutical School Jiangnan University, Wuxi 214122, Chin)
出处
《广东化工》
CAS
2018年第14期34-36,26,共4页
Guangdong Chemical Industry
基金
国家自然科学基金(21574059)
江苏省自然科学基金(BK 20170202)
