抑制容器荧光干扰的双轴共焦拉曼检测方法

A Dual-Axis Confocal Raman Detection Method for Suppressing Fluorescence Interference from Containers

为消除拉曼光谱检测中样品装载容器的荧光背景干扰,利用样品拉曼信号光与容器产生的荧光不共焦的特性,设计了一种双轴共焦的检测光路结构。实验结果表明:来自容器的荧光干扰幅度出现数量级的衰减,拉曼信号光幅度下降了约30%;双轴共焦拉曼检测方法能够避免传统共轴共焦检测方法中拉曼信号光和荧光混合接收的问题,解决了荧光干扰对拉曼信号动态检测范围的限制,实现了对荧光材料容器装载样品拉曼信号的有效检测。

Aimed at eliminating the fluorescence interference from containers of the sample in the detection of Raman spectroscopy,a dual-axis confocal detection scheme is proposed based on the characteristic that the fluorescence generated by the container and the Raman signal of the sample are not confocal. The experimental results show that the fluorescence interference signals from the container are reduced by an order of magnitude,and the intensity of Raman signal decreases by about 30%. The dual-axis confocal Raman detection scheme can solve the mixed reception problem of Raman signal and fluorescence in the conventional coaxial confocal Raman detection scheme,solve the limitation of the dynamic detection range of Raman signal caused by the fluorescence interference,and achieve the effective detection of Raman signal of the material in fluorescent material containers.