补骨脂素抗抑郁作用及机制研究

Study on the anti-depressant effect of psoralen and its mechanism

目的研究补骨脂素的抗抑郁作用及机制。方法采用大鼠强迫游泳、小鼠强迫游泳及小鼠悬尾3种抑郁模型，将动物随机分为对照组、补骨脂素低剂量组、补骨脂素高剂量组、阿米替林组，观察各组药物对大／小鼠强迫游泳不动时间和悬尾不动时间的影响；同时在细胞水平建立皮质酮损伤PC12细胞模型，观察补骨脂素的细胞保护作用。结果在大／小鼠强迫游泳和小鼠悬尾实验中，补骨脂素可显著缩短大／小鼠的强迫游泳不动时间和悬尾不动时间，与对照组比较差异均有统计学意义（P〈0．05）；皮质酮损伤PCI2细胞模型中，补骨脂素显著提高PC12细胞的存活率，拈抗皮质酮诱导的细胞损伤作用。结论补骨脂素具有抗抑郁效应，其机制可能与神经细胞保护作用相关。

Objective To investigate the anti-depressant effects of psoralen and its mechanisms. Methods The anti-depressant effects of psoralen were evaluated by the forced swimming tests in rats and mice and the tail suspension test in mice. The animals were randomly divided into four groups： control group, low dose psoralen group, high close psoralen group and amitriptyline group. The effects of psoralen on immobility time were measured in the forced swimming test and tail suspension test in rats and mice. Likewise, PC12 cells injury was induced by corticosterone and the effects of psoralen on the cell viability were measured by MT1r assay. Results Psoralen significantly decreased the immobility time in the forced swimming tests and tail suspension test in rats and mice, which were significantly different compared with the control group （P 〈 0.05）. In addition, psoralen could improve the survival rate of PC12 cells injuried by cortieosterone. Conclusions Psoralen has anti-depressant effects and its mechanisms may be involved in the protective effects of neural cells.