摘要
[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.
[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis （AA） rats and its mechanism from cytokine levels.[Methods] The complete Freund s adjuvant （CFA）-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling, arthritis score, immune organ index and hematoxylin-eosin （HE） staining. The content of inflammatory factors TNF-α, IL-6, and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay （ELISA） respectively.[Results] Compared with the model group, the total extract of K. coccinea （KCTE） could significantly inhibit the primary and secondary articular swelling of AA rats, lower the arthritis score and immune organ index, and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE （KCTE-H and KCTE-M）, KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats, exhibiting a certain dose-effect relationship.[Conclusions] KCTE had significant therapeutic effect on the AA of rats, and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.
基金
Supported by National Natural Science Foundation of China(No.81303162)
China Postdoctoral Science Foundation(No.2015T80784,2014M552017)
Postdoctoral Scientific Research Project of Henan Province(No.2013010)
