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Caco-2细胞培养、细胞单层模型建立的研究 被引量:3

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摘要 近年来,研究药物在细胞层面的活动情况,比较常用的工具是Caco-2细胞模型[1-2]。Caco-2细胞培养成熟后与人体小肠的上皮细胞相似[2]。本研究中,将Caco-2细胞接种于TranswellTM小室中,建立Caco-2细胞单层模型,可用于研究中枢神经系统药物与P-gp底物跨膜转运的相互影响。1仪器与试剂1.1仪器低温高速离心机:Beckman,美国;倒置相差显微镜(BCM-1000):Nikon,日本。
出处 《临床合理用药杂志》 2018年第20期161-162,共2页 Chinese Journal of Clinical Rational Drug Use
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