摘要
目的:基于高效液相指纹图谱和光谱-抗肿瘤作用的关系来区别两种斑蝥.方法:在本研究中,开发了一种简单有效的高效液相色谱法整合化学计量学分析与光谱-抗肿瘤作用关系的方法来区别两种斑蝥:南方大斑蝥(MP)和黄黑小斑蝥(MC).结果:在指纹图谱分析中,我们通过PCA和OPLS一DA用相似性和模式识别分析的选择了14个特征峰来评估MP与MC的差异.来自中国10个地区样本的HPLC色谱图表明了MP和MC之间的差异,另外,发现了7个化学标志物.在光谱-抗肿瘤作用关系分析中,通过灰度相关分析和多元线性回归分析,发现了4个活性标志物能够降低IC50,可能是斑蝥中的抗肿瘤成分.化学计量分析法联合光谱-效应关系的结果表明峰2(胞嘧啶),4(未知)和14(未知)是区分2种斑蝥的重要区分标志物.结论:这种方法可行,可信并且能有效的区分MP和MC,并能提供一种对昆虫药物的质量控制的新方法.
Objective: Evaluation of discrimination between two Mylabris Species based on HPLC fingerprinting andspectrum-antitumor effect relationship. Methods: In this study, a simple and efficient high-performance liquidchromatography (HPLC) method integrating with chemometric analysis and spectrum-antitumor effect relationship wasdeveloped for discrimination between two species of Mylabris: Mylabris phalerata Pallas (MP) and Mylabris cichoriiLinnaeus (MC). Results: In the fingerprint analysis, 14 characteristic peaks were selected to assess the differencesbetween MP and MC using the similarity and pattern recognition analysis using PCA and OPLS-DA. The HPLCchromatograms of samples from 10 regions of China showed differences between MP and MC, and 7 characteristicchemical markers were found. In the spectrum-antitumor effect relationship analysis, 4 activity markers played a vitalrole in decreasing the IC50 and might be the antitumor components of Mylabris by grey relational analysis andmultivariate linear regression analysis. The chemometric analysis in combination with spectrum-effect relationshipresults indicated that peaks 2 (cytosine), 4 (unknown) and 14 (unknown) were important differential markers fordistinguishing the two species of Mylabris. Conclusion: The method is applicable, credible and more efficient todiscriminate MP and MC, and will offer a new way for facilitating quality control of insect medicines.
基金
Funding: This study was funded by the National Natural Science Foundation of China (81260488 and 81560669), KeyLab Construction Project of the Educational Department of Guizhou Province (Project No. Guizhou EducationCooperation KY[2014]212) , Science Inovative Talent Team for Medicinal Insect Research and Development in Zunyi(Zunyi shi ke he 2015-40) and Modernization of Traditional Chinese Medicine in Guizhou Province high-tech researchand development projects (Qian ke he ZY 2012-2009).